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MABS1964-100UG Anti-PEG Antibody, clone AGP4

Anti-PEG, clone AGP4, Cat. No. MABS1964, is a mouse monoclonal antibody that detects Polyethylene glycol (PEG) and has been tested for use in ELISA, Western Blotting, and Immunohistochemistry.

MABS1964-100UG
100 μg  
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Áttekintés

Replacement Information
Description
Catalogue NumberMABS1964-100UG
DescriptionAnti-PEG Antibody, clone AGP4
Alternate Names
  • Polyethylene Glycol
Background InformationPolyethylene glycols (PEG) are non-toxic, uncharged, hydrophilic long chain polymers with a big exclusion volume in aqueous solution. The covalent attachment of PEG is commonly employed to modify proteins and drugs that can increase their bioavailability and reduce immunogenicity and proteolytic degradation. PEG is considered as a non-immunogenic moiety and has been widely tested for its ability to protect proteins from the host s immune system. This antibody (Clone AGP4) binds to the PEG backbone and is suitable for capture of PEGylated compounds in ELISA as well as the detection of PEGylated molecules. It is suitable for sensitive ELISA applications where AGP4 can be used as a capture antibody. Note: Tween-20 should be used in wash buffers for this antibody combination.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgM in PBS with 0.04% sodium azide.
Applications
ApplicationAnti-PEG, clone AGP4, Cat. No. MABS1964, is a mouse monoclonal antibody that detects Polyethylene glycol (PEG) and has been tested for use in ELISA, Western Blotting, and Immunohistochemistry.
Key Applications
  • ELISA
  • Immunohistochemistry
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A representative lot detected PEG in Immunohistochemistry applications (Wang, H., et. al. (2012). J Virol. 86(11):6286-302).

Western Blotting Analysis: A representative lot detected PEG in Western Blotting applications (Ehrlich, G.K., et. al. (2009). J Mol Recognit. 22(2):99-103).

ELISA Analysis: A representative lot detected PEG in ELISA applications (Hao WR et al (2018). Sci. Rep. 8(1); 4256; Chang, C.J., et. al. (2017). Nat Commun. 8(1); 522; Henry, C.E., et. al. (2016). Acta Biomater. 43:61-70).
Biological Information
ImmunogenPolyethylene glycol-conjugated to E.coli beta-glucuronidase.
CloneAGP4
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone AGP4 specifically detects proteins conjugated with low to high molecular weight methoxy and amino polyethylene glycols (PEGs).
IsotypeIgMκ
Species Reactivity
  • All
Species Reactivity NoteAll.
Antibody TypeMonoclonal Antibody
Purification MethodPurified by ion-exchange chromatography
Molecular WeightN/A
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by ELISA with amino- and methoxy PEG molecules (10K-PEG, 3K-PEG, and 2K-mPEG).

ELISA Analysis: 5 µg/mL of this antibody detected amino- and methoxy-PEG molecules (10K amino-PEG, 3K-amino PEG, and 2K-methoxy PEG).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABS1964-100UG 04054839613821