Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

CBL1337 Anti-PECAM-1 Antibody, clone 390

CBL1337
500 µg  
Purchase on Sigma-Aldrich

Különleges ajánlatok

Áttekintés

Különleges ajánlatok

Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
MFC, IHC, IP, FUNCRPurifiedMonoclonal Antibody
Description
Catalogue NumberCBL1337
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-PECAM-1 Antibody, clone 390
Alternate Names
  • CD31
Background InformationCD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1) is a type I integral membrane glycoprotein, and a member of the immunoglobulin superfamily of cell surface receptors. PECAM-1 is constitutively expressed on the surface of endothelial cells where it is concentrated at cell junctions. PECAM-1 is also expressed at low levels on all leukocytes and platelets. Homotypic interaction of CD31 is important in cell-cell interaction, cell-matrix interaction and signal transduction.
Product Information
FormatPurified
Control
  • Jurkat and Raji cells, tonsil tissue
PresentationProtein A Purified mouse immunoglobulin in 100mM borate buffered saline, pH 8.0, containing no preservative.
Quality LevelMQ100
Applications
ApplicationAnti-PECAM-1 Antibody, clone 390 is an antibody against PECAM-1 for use in FC, IH & IP.
Key Applications
  • Flow Cytometry
  • Immunohistochemistry
  • Immunoprecipitation
  • Affects Function
Application NotesFlow Cytometry: 1 μg/10e6 cells.

Immunohistochemistry: acteone fixed frozen tissue sections

Immunoprecipitation

Blocking of cell adhesion: CBL1337 inhibits aggregation of L cells transfected with a variant form of CD31 (DeLisser, 1993)

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenMouse leukocyte cell line 32D
Clone390
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRat
SpecificityCBL1337 recognizes mouse PECAM-1, a 130-140 kDa type 1 integral membrane glycoprotein. This antibody has been shown to inhibit the aggregation of L cells transfected with a variant form of PECAM-1 (DeLisser, 1993).
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PECAM1
  • EndoCAM
  • GPIIA
  • PECAM-1
  • CD31
  • CD31/EndoCAM
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P16284 # This protein is a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions.
SIZE: 738 amino acids; 82536 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Long isoform predominates all tissues examined, isoform Delta12 was detected only in trachea and isoform Delta14-15 only in lung, isoform Delta14 was detected in all tissues examined with the strongest expression in heart.
PTM: Phosphorylated on Ser and Tyr residues after cellular activation.
SIMILARITY: SwissProt: P16284 ## Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size500 µg
Global Trade Item Number
Katalógusszám GTIN
CBL1337 04053252739774