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ST1537 Anti-PCSK1 Mouse mAb

Anti-PCSK1 Mouse mAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Proprotein Convertase Subtilisin/Kexin 1

ST1537
  
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M
Description
Overview

This product has been discontinued.





Recognizes the ~80 kDa PCSK1 protein in HeLa, PC12, Raw 264.7, and NIH3T3 cells and human small intestine tissue.
Catalogue NumberST1537
Brand Family Calbiochem®
SynonymsAnti-Proprotein Convertase Subtilisin/Kexin 1
Application Data
Detection of human PCSK1 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (1-5 µg/ml). Detection: chemiluminescence.

Detection of rat PCSK1 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (1-5 µg/ml). Detection: chemiluminescence.

Detection of mouse PCSK1 by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (1-5 µg/ml). Detection: chemiluminescence.

Detection of mouse PCSK1 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human PCSK1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human small intestine tissue. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (3 µg/ml). Detection: colorimetric (DAB).

Detection of human PCSK1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldahyde. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (30 µg/ml). Detection: fluorescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlHeLa cells, PC12 cells, Raw 264.7 cells, NIH3T3 cells, or Human small intestine tissue
PreservativeNone
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Paraffin Sections (3 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids 652-754 of human PCSK1, expressed as a GST fusion protein
HostMouse
IsotypeIgG₃
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
ST1537 0

Documentation

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-November-2010 RFH
SynonymsAnti-Proprotein Convertase Subtilisin/Kexin 1
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Paraffin Sections (3 µg/ml)
Application Data
Detection of human PCSK1 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (1-5 µg/ml). Detection: chemiluminescence.

Detection of rat PCSK1 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (1-5 µg/ml). Detection: chemiluminescence.

Detection of mouse PCSK1 by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (1-5 µg/ml). Detection: chemiluminescence.

Detection of mouse PCSK1 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human PCSK1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human small intestine tissue. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (3 µg/ml). Detection: colorimetric (DAB).

Detection of human PCSK1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldahyde. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (30 µg/ml). Detection: fluorescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~80 kDa PCSK1 protein.
BackgroundThe members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This encoded protein is a type I proinsulin-processing enzyme that plays a key role in regulating insulin biosynthesis. It is also known to cleave proopiomelanocortin, prorenin, proenkephalin, prodynorphin, prosomatostatin and progastrin. Mutations in this gene are thought to cause obesity.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids 652-754 of human PCSK1, expressed as a GST fusion protein
IsotypeIgG₃
Specieshuman, mouse, rat
Positive controlHeLa cells, PC12 cells, Raw 264.7 cells, NIH3T3 cells, or Human small intestine tissue
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review