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MABF2122-100UG Anti-PCBP2 (hnRNP E2) Antibody, clone PC81-1

Anti-PCBP2 (hnRNP E2), clone PC81-1, Cat. No. MABF2122, is a mouse monoclonal antibody that detects Poly(rC)-binding protein 2 and has been tested for use in Immunocytochemistry and Western Blotting.

Anti-PCBP2 (hnRNP E2) Antibody, clone PC81-1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: PCBP2 UniProt Number: Q15366
MABF2122-100UG
100 μg  
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Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF2122-100UG
DescriptionAnti-PCBP2 (hnRNP E2) Antibody, clone PC81-1
Alternate Names
  • Poly(rC)-binding protein 2
  • Alpha-CP2
  • Heterogeneous nuclear ribonucleoprotein E2
  • hnRNP E2
Background InformationPoly(rC)-binding protein 2 (UniProt: Q15366; also known as Alpha-CP2, Heterogeneous nuclear ribonucleoprotein E2, hnRNP E2) is encoded by the PCBP2 gene (Gene ID: 5094) in human. PCBP2 belongs to a class of cellular proteins that bind to poly(C) stretches of both RNA and DNA and has been detected in all tissues studied. PCBP2 shuttles between the nucleus and cytoplasm and exerts its impact on RNA processing and mRNA expression through sequence-specific interactions with C-rich determinants within target mRNAs. It acts as an important mediator of multiple processes, including replication of viruses with gastrointestinal and hepatic tropism, hepatic collagen synthesis, globin expression, and cellular proliferation. PCBP2 is shown to negatively regulate cellular antiviral responses mediated by mitochondrial antiviral-signaling protein (MAVS) signaling and acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, thereby triggering MAVS ubiquitination and degradation. Together with PCBP1, it is shown to function as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. PCBP2 contains three K-homologous (KH) domains that mediates poly(C) binding. Eight different isoforms of PCBP2 have been described that are produced by alternative splicing. (Ref.: Ghanem, LR et al. (2014). Gene Exp. Patterns 14(2); 78-87; Sean, P., et al. (2008). Virology. Virology. 378(2); 243-253).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-PCBP2 (hnRNP E2), clone PC81-1, Cat. No. MABF2122, is a mouse monoclonal antibody that detects Poly(rC)-binding protein 2 and has been tested for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected PCBP2 (hnRNP E2) in Western Blotting applications (Sean, P., et. al. (2008). Virology. 378(2):243-53; Chase, A.J., et. al. (2014). Virology. 449:35-44).

Immunocytochemistry Analysis: A representative lot detected PCBP2 (hnRNP E2) in Immunocytochemistry applications (Fitzgerald, K.D., et. at. (2011). PLoS Pathog. 7(7):e1002127).

Western Blotting Analysis: 1 µg/mL from a representative lot detected PCBP2 (hnRNP E2) in SK-N-SH cell lysate.
Biological Information
ImmunogenHis-tagged full-length human recombinant Poly(rC)-binding protein 2.
ClonePC 81-1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone PC 81-1 specifically detects Poly(rC)-binding protein 2 in human cells.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PCBP2
Purification MethodProtein G purified
UniProt Number
Molecular Weight~38 kDa observed; 38.58 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected PCBP2 (hnRNP E2) in HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABF2122-100UG 04054839563324