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MABS1188 Anti-PAFAH 1B3 Antibody, clone 3E4.1

Anti-PAFAH 1B3 Antibody, clone 3E4.1 is an antibody against PAFAH 1B3 for use in Western Blotting, Immunocytochemistry.

Anti-PAFAH 1B3 Antibody, clone 3E4.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Lipid Signaling Gene Symbol: PAFAH1B3, PAFAHG UniProt Number: Q15102
MABS1188
100 μg  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1188
DescriptionAnti-PAFAH 1B3 Antibody, clone 3E4.1
Alternate Names
  • Platelet-activating factor acetylhydrolase IB subunit gamma
  • PAF acetylhydrolase 29 kDa subunit
  • PAF-AH 29 kDa subunit
  • PAF-AH1b subunit alpha 1
  • PAF-AH subunit gamma
  • PAFAH subunit gamma
Background InformationPlatelet-activating factor acetylhydrolase IB subunit gamma (EC 3.1.1.47; UniProt Q15102; also known as PAF acetylhydrolase 29 kDa subunit, PAF-AH 29 kDa subunit, PAF-AH1b subunit alpha 1, PAF-AH subunit gamma, PAFAH subunit gamma) is encoded by the PAFAH1B3 (also known as PAFAHG) gene (Gene ID 5050) in human. Platelet-activating factor (PAF) acetylhydrolase (PAF-AH) negatively regulates PAF potency by catalyzing the removal of the acetyl group at the sn-2 position of the lipid messenger. There are two types of intracellular PAF-AH and a plasma type PAF-AH. Type I intracellular PAF-AH is a heterotrimeric G-protein-like complex composed of alpha1 (originally called gamma), alph2 (originally called alpha), and beta subunits. Both alpha1 and alpha2 contains a catalytic site. The alpha2/2 homodimer hydrolyzes PAF (1-O-alkyl-2-acetyl-sn-glycero-3-PC) and 1-O-alkyl-2-acetyl-sn-glycero-3-PE more efficiently, while the alpha1/1 homodimer and alpha1/2 heterodimer are more efficient toward 1-O-alkyl-2-acetyl-sn-glycero-3-PA than PAF. The alpha 1 subunit is reported to be up-regulated in primary human breast tumors and correlated with poor prognosis. Inactivation of alpha 1 subunit enhances cellular level of tumor-suppressing signaling lipids.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-PAFAH 1B3 Antibody, clone 3E4.1 is an antibody against PAFAH 1B3 for use in Western Blotting, Immunocytochemistry.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:200 dilution from a representative lot detected PAFAH1B3 in A431, HeLa, HUVECs and NIH/3T3 cells.
Biological Information
ImmunogenGST-tagged recombinant internal fragment from the C-terminal half of human PAFAH1B3.
EpitopeInternal (C-terminal half).
Clone3E4.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 3E4.1 detects the alpha 1 subunit found in the heterotrimeric type I intracellular PAF-AH. The alpha 1 subunit was originally named the gamma subunit (Arai, H., et al. (2002) J. Biochem. 131(5):635-640; Hattori, M., et al. (1993) J. Biol. Chem. 268(25):18748-18753).
IsotypeIgG2bκ
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse. Predicted to react with Rat, Bovine based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PAFAH1B3
  • PAFAHG
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~30 kDa observed. 25.73 kDa calculated. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PAFAH1B3 in 10 µg of K562 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABS1188 04055977332209