Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

487924 Anti-Nitrotyrosine Rabbit pAb

Anti-Nitrotyrosine Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
487924
  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes nitrotyrosine.
Catalogue Number487924
Brand Family Calbiochem®
Application Data
Detection of bovine nitrotyrosine by immunoblotting. Samples: Low molecular weight standards (lane 1), BSA (lane 2), peroxynitrite-treated BSA (lane 3 and 4), peroxynitrite-treated cell lysate (lane 5). Primary antibody: Anti-Nitrotyrosine Rabbit pAb (Cat. No. 487924) (Assay Dependent on Primary Anitbody). Detection: chemiluminescence.
References
ReferencesMyatt, L., et al. 1996. Hypertension 28, 488.
Beckman, J.S., et al. 1994. Biol. Chem. Hoppe-Seyler 375, 81.
Product Information
FormLiquid
FormulationIn TBS, 0.5 mg/ml BSA, 50% glycerol, pH 7.4.
Negative controlBSA (untreated)
Positive controlCells treated with peroxynitrite
Preservative≤0.1% sodium azide
ReconstitutionReconstitute with 500 μl H₂O.
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1-2 µg/ml)
Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogenperoxynitrite-treated KLH
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
487924 0

Documentation

Anti-Nitrotyrosine Rabbit pAb Certificates of Analysis

TitleLot Number
487924

References

Hivatkozások áttekintése
Myatt, L., et al. 1996. Hypertension 28, 488.
Beckman, J.S., et al. 1994. Biol. Chem. Hoppe-Seyler 375, 81.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-August-2007 RFH
ApplicationImmunoblotting (1-2 µg/ml)
Application Data
Detection of bovine nitrotyrosine by immunoblotting. Samples: Low molecular weight standards (lane 1), BSA (lane 2), peroxynitrite-treated BSA (lane 3 and 4), peroxynitrite-treated cell lysate (lane 5). Primary antibody: Anti-Nitrotyrosine Rabbit pAb (Cat. No. 487924) (Assay Dependent on Primary Anitbody). Detection: chemiluminescence.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes nitrotyrosine-containing proteins.
BackgroundNitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by Nitric oxide synthase. The reaction of NO with superoxide which is often present under conditions of oxidative stress, forms peroxynitrite, a strong oxidant which can nitrosate tyrosine residues on proteins. Nitrotyrosine has been shown to be present in proteins from artherosclerotic lesions of human carotid arteries and also in placenta during pre-eclampsia.
HostRabbit
Immunogenperoxynitrite-treated KLH
IsotypeIgG
Speciesa broad range of species
Positive controlCells treated with peroxynitrite
Negative controlBSA (untreated)
FormLiquid
FormulationIn TBS, 0.5 mg/ml BSA, 50% glycerol, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsVariables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesMyatt, L., et al. 1996. Hypertension 28, 488.
Beckman, J.S., et al. 1994. Biol. Chem. Hoppe-Seyler 375, 81.