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482736 Anti-Nitric Oxide Synthase (2-12), Endothelial N-Terminal Rabbit pAb

Anti-Nitric Oxide Synthase (2-12), Endothelial N-Terminal Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-eNOS

482736
  
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Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~135 kDa myristoylated and non-myristoylated forms of eNOS.
Catalogue Number482736
Brand Family Calbiochem®
SynonymsAnti-eNOS
References
Product Information
FormLyophilized
FormulationLyophilized undiluted serum.
PreservativeNone
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesImmunoblotting (1:800)
Immunocytochemistry (1:400)
Application CommentsDoes not cross-react with recombinant human inducible NOS (iNOS) or neuronal NOS (nNOS). Variables associated with assay conditions will dictate the proper working dilution.

Recommended Procedure for Immunoblotting of Anti-eNOS:

1. Run SDS-PAGE using 4-15% gradient gels or single percentage gels (e.g., 7.5%) and electrophoretically transfer to PVDF membrane.

2. Block overnight with 4% normal goat serum in evaporated goat milk diluted 1:5 with TBS/Tween®-20 detergent buffer.

3. Wash 2X with TBS/Tween®-20 detergent.

4. Apply the reconstituted rabbit anti-eNOS at a dilution of at least 1:500 in the following diluent: 1% normal goat serum in evaporated goat milk diluted 1:5 with TBS/Tween®-20 detergent buffer. Higher dilutions of the primary antibody may be required. Incubate 2-4 h.

5. Wash 3X with TBS/Tween®-20 detergent.

6. Apply HRP-conjugated, affinity-purified goat anti-rabbit IgG at the recommended dilution. Use 1% normal goat serum in evaporated goat milk diluted 1:5 with TBS/Tween®-20 detergent as the diluent. Incubate 1-2 h.

7. Wash 3X with TBS/Tween®-20 detergent, then soak overnight in a reasonably large volume of the same buffer.

8. Develop the blot using an HRP substrate suitable for immunoblotting, such as DAB.
Biological Information
Immunogena synthetic peptide corresponding to amino acids 2-12 of human endothelial nitric oxide synthase (eNOS)
ImmunogenHuman
HostRabbit
IsotypeIgG
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsReconstitute with 100 µl PBS, 1% BSA. Following reconstitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solution.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
482736 0

Documentation

Anti-Nitric Oxide Synthase (2-12), Endothelial N-Terminal Rabbit pAb Certificates of Analysis

TitleLot Number
482736
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-August-2007 RFH
SynonymsAnti-eNOS
ApplicationImmunoblotting (1:800)
Immunocytochemistry (1:400)
DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~135 kDa endothelial nitric oxide synthase (eNOS) protein.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids 2-12 of human endothelial nitric oxide synthase (eNOS)
IsotypeIgG
Specieshuman
FormLyophilized
FormulationLyophilized undiluted serum.
PreservativeNone
CommentsDoes not cross-react with recombinant human inducible NOS (iNOS) or neuronal NOS (nNOS). Variables associated with assay conditions will dictate the proper working dilution.

Recommended Procedure for Immunoblotting of Anti-eNOS:

1. Run SDS-PAGE using 4-15% gradient gels or single percentage gels (e.g., 7.5%) and electrophoretically transfer to PVDF membrane.

2. Block overnight with 4% normal goat serum in evaporated goat milk diluted 1:5 with TBS/Tween®-20 detergent buffer.

3. Wash 2X with TBS/Tween®-20 detergent.

4. Apply the reconstituted rabbit anti-eNOS at a dilution of at least 1:500 in the following diluent: 1% normal goat serum in evaporated goat milk diluted 1:5 with TBS/Tween®-20 detergent buffer. Higher dilutions of the primary antibody may be required. Incubate 2-4 h.

5. Wash 3X with TBS/Tween®-20 detergent.

6. Apply HRP-conjugated, affinity-purified goat anti-rabbit IgG at the recommended dilution. Use 1% normal goat serum in evaporated goat milk diluted 1:5 with TBS/Tween®-20 detergent as the diluent. Incubate 1-2 h.

7. Wash 3X with TBS/Tween®-20 detergent, then soak overnight in a reasonably large volume of the same buffer.

8. Develop the blot using an HRP substrate suitable for immunoblotting, such as DAB.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute with 100 µl PBS, 1% BSA. Following reconstitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solution.
Toxicity Standard Handling