Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

AB9240 Anti-NeuroD 6 Antibody

This Anti-NeuroD 6 Antibody is validated for use in WB, IH for the detection of NeuroD 6.

Anti-NeuroD 6 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience, Neuronal & Glial Markers Gene Symbol: NEUROD6, MATH2, Math-2, ATOH2, NeuroD6, NEX1M, Atoh2 UniProt Number: Q96NK8
AB9240
100 µg  
Purchase on Sigma-Aldrich

Különleges ajánlatok

Áttekintés

Replacement Information

Különleges ajánlatok

100% Performance Guaranteed

Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB9240
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-NeuroD 6 Antibody
OverviewTo reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not effect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

Resuspend the antibody pellet in any suitable biological buffer, standard PBS or TBS (pH 7.3-7.5) are typical. Volumes required are not critical but it is suggested that the final antibody concentration be between 0.1 mg/mL and 1.0 mg/mL. For example, to achieve a 1 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25C° prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.
Alternate Names
  • MATH2
  • NEX1
References
Product Information
FormatAffinity Purified
PresentationAffinity purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
Quality LevelMQ100
Applications
ApplicationThis Anti-NeuroD 6 Antibody is validated for use in WB, IH for the detection of NeuroD 6.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern blot: 1:200-1:1,000 using ECL.

Immunohistochemistry: 1:200-1:1,000.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenSynthetic peptide.
HostRabbit
SpecificityRecognizes NeuroD 6 (MATH2, NEX1), a Helix-loop-helix class of transcription factor.
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryNEUROD6 is a member of the NEUROD (NEUROD1; MIM 601724) family of basic helix-loop-helix (bHLH) transcription factors (Guo et al., 2002 [PubMed 12357074]).[supplied by OMIM]
Gene Symbol
  • NEUROD6
  • MATH2
  • Math-2
  • ATOH2
  • NeuroD6
  • NEX1M
  • Atoh2
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q96NK8 # Activates E box-dependent transcription in collaboration with TCF3/E47. May be a trans-acting factor involved in the development and maintenance of the mammalian nervous system. Transactivates the promoter of its own gene (By similarity).
SIZE: 337 amino acids; 38705 Da
SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein (By similarity).
SUBCELLULAR LOCATION: Nucleus (Probable).
SIMILARITY: SwissProt: Q96NK8 ## Contains 1 basic helix-loop-helix (bHLH) domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain unopened vial at -70C° for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

The rehydrated antibody solutions can be stored undiluted at 2-8C° for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8C°.

For storage at -20C°, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70C° for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
AB9240 04053252584060