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AB5032 Anti-Neural Cell Adhesion Molecule Antibody

AB5032
50 µg  
Purchase on Sigma-Aldrich

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Áttekintés

Replacement Information

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Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
Ch, H, M, RELISA, IF, IHC, FUNC, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5032
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Neural Cell Adhesion Molecule Antibody
Alternate Names
  • CD56 antigen
  • antigen recognized by monoclonal antibody 5.1H11
Background InformationThe Immunoglobulin superfamily of cell adhesion molecules is related to the immunoglobulins proteins by structure. The family contains over a 100 members, which include ICAM (intercellular adhesion molecule), NCAM (neural cell adhesion molecule) and NgCAM (neural-glial cell adhesion molecule). Both NCAM and NgCAM are calcium independent adhesion molecules. NCAM, the first CAM to be characterized, is expressed on all neurons from very early in development. It binds homophilically to NCAM on adjacent cells and has a variety of functions during neural development. One of these important functions is to hold developing neurites together during neuron outgrowth and formation of neural connections. The adhesive properties of NCAM are modulated during development and plasticity by the presence of the regulated carbohydrate, polysialic acid (PSA). The post-translational addition of alpha-2,8-linked PSA (NCAM-PSA) appears to contribute to synaptic plasticity during learning and memory formation and to the mechanisms of nerve regeneration.
References
Product Information
FormatAffinity Purified
HS Code3002 15 90
Control
  • Neuraminidase-treated mouse brain membrane.
PresentationPurified rabbit polyclonal in buffer containing PBS containing no preservatives.
Quality LevelMQ100
Applications
ApplicationDetect Neural Cell Adhesion Molecule using this Anti-Neural Cell Adhesion Molecule Antibody validated for use in ELISA, IF, IH, FUNC & WB.
Key Applications
  • ELISA
  • Immunofluorescence
  • Immunohistochemistry
  • Affects Function
  • Western Blotting
Application NotesImmunohistochemistry:
A previous lot was shown to work with a wide range of fixatives. Will detect chicken NCAM using a concentration of 1 μg/mL and mouse NCAM using a concentration of 2 μg/mL.

Immunofluorescence:
A previous lot of this antibody was used in Immunofluorescence.

Function blocking:
A previous lot of this antibody was used in Function blocking.

ELISA:
0.3 μg/mL of a previous lot was used.

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenHighly purified chicken NCAM
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityNeural Cell Adhesion Molecule (NCAM). MW is 200-250 kDa; when treated with Neuraminidase, 140-180 kDa. A breakdown product of 160 kDa may also be visible.
Species Reactivity
  • Chicken
  • Human
  • Mouse
  • Rat
Species Reactivity NoteMouse. Expected to cross-react with Chicken, Human, and rat.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CD56
  • NCAM
  • MSK39
  • NCAM1
Purification MethodImmunoAffinity Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P13592 # This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.| P13591 # This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
SIZE: 761 amino acids; 83770 Da
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor.
DOMAIN: SwissProt: P13592
SIMILARITY: SwissProt: P13592 ## Contains 2 fibronectin type-III domains. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains. | P13591 ## Contains 2 fibronectin type-III domains. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Molecular WeightMW is 200-250 kDa; when treated with Neuraminidase, 140-180 kDa. A breakdown product of 160 kDa may also be visible.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on mouse brain lysates.

Western Blot Analysis: 1:1000 dilution of this lot detected NCAM on 10 μg of mouse brain lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
AB5032 08436037122514

Documentation

Anti-Neural Cell Adhesion Molecule Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-Neural Cell Adhesion Molecule Antibody Certificates of Analysis

TitleLot Number
Anti-Neural Cell Adhesion Molecule 2475623
Anti-Neural Cell Adhesion Molecule - 2383466 2383466
Anti-Neural Cell Adhesion Molecule - 2420359 2420359
Anti-Neural Cell Adhesion Molecule - 2453132 2453132
Anti-Neural Cell Adhesion Molecule - 1969095 1969095
Anti-Neural Cell Adhesion Molecule - 2002167 2002167
Anti-Neural Cell Adhesion Molecule - 2089395 2089395
Anti-Neural Cell Adhesion Molecule - 2167131 2167131
Anti-Neural Cell Adhesion Molecule - 2299534 2299534
Anti-Neural Cell Adhesion Molecule - 3208811 3208811

References

Reference overviewApplicationSpeciesPub Med ID
Why are enteric ganglia so small? Role of differential adhesion of enteric neurons and enteric neural crest cells.
Rollo, BN; Zhang, D; Simkin, JE; Menheniott, TR; Newgreen, DF
F1000Research  4  113  2015

Kivonat megmutatása
26064478 26064478
Transient appearance of the epithelial invagination in the olfactory pit of chick embryos.
Nakamuta, S; Nakamuta, N; Yamamoto, Y; Onodera, N; Araki, I
The Journal of veterinary medical science / the Japanese Society of Veterinary Science  77  89-93  2015

Kivonat megmutatása
Immunohistochemistry25231436 25231436
Dehydroevodiamine·HCl Improves Stress-Induced Memory Impairments and Depression Like Behavior in Rats.
Kim, HJ; Shin, KY; Chang, KA; Ahn, S; Choi, HS; Kim, HS; Suh, YH
The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology  18  55-9  2014

Kivonat megmutatása
Western Blotting24634597 24634597
High-resolution array CGH profiling identifies Na/K transporting ATPase interacting 2 (NKAIN2) as a predisposing candidate gene in neuroblastoma.
Romania, P; Castellano, A; Surace, C; Citti, A; De Ioris, MA; Sirleto, P; De Mariano, M; Longo, L; Boldrini, R; Angioni, A; Locatelli, F; Fruci, D
PloS one  8  e78481  2013

Kivonat megmutatása
Western BlottingHuman24205241 24205241
Polysialylated NCAM and ephrinA/EphA regulate synaptic development of GABAergic interneurons in prefrontal cortex.
Brennaman, LH; Zhang, X; Guan, H; Triplett, JW; Brown, A; Demyanenko, GP; Manis, PB; Landmesser, L; Maness, PF
Cerebral cortex (New York, N.Y. : 1991)  23  162-77  2013

Kivonat megmutatása
22275477 22275477
Testosterone improves the regeneration of old and young mouse skeletal muscle.
Serra, C; Tangherlini, F; Rudy, S; Lee, D; Toraldo, G; Sandor, NL; Zhang, A; Jasuja, R; Bhasin, S
The journals of gerontology. Series A, Biological sciences and medical sciences  68  17-26  2013

Kivonat megmutatása
22499765 22499765
Artemin, a glial cell line-derived neurotrophic factor family member, induces TRPM8-dependent cold pain.
Lippoldt, EK; Elmes, RR; McCoy, DD; Knowlton, WM; McKemy, DD
The Journal of neuroscience : the official journal of the Society for Neuroscience  33  12543-52  2013

Kivonat megmutatása
23884957 23884957
Delta-like 1 homolog (dlk1): a marker for rhabdomyosarcomas implicated in skeletal muscle regeneration.
Jørgensen, LH; Sellathurai, J; Davis, EE; Thedchanamoorthy, T; Al-Bader, RW; Jensen, CH; Schrøder, HD
PloS one  8  e60692  2013

Kivonat megmutatása
23577150 23577150
Integrins and cAMP mediate netrin-induced growth cone collapse.
Lemons, ML; Abanto, ML; Dambrouskas, N; Clements, CC; Deloughery, Z; Garozzo, J; Condic, ML
Brain research  1537  46-58  2013

Kivonat megmutatása
24001590 24001590
Process-based expansion and neural differentiation of human pluripotent stem cells for transplantation and disease modeling.
Stover, AE; Brick, DJ; Nethercott, HE; Banuelos, MG; Sun, L; O'Dowd, DK; Schwartz, PH
Journal of neuroscience research  91  1247-62  2013

Kivonat megmutatása
Immunocytochemistry23893392 23893392