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SC1000 Anti-Nanog Rabbit pAb

This Anti-Nanog Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation for the detection of Nanog.

Anti-Nanog Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Nanog Homeobox, Anti-ENK, Anti-Early Embryo Specific Expression NK Family Gene

SC1000
  
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Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Host
Rb
Description
OverviewRecognizes the ~40 kDa Nanog protein in mouse myeloid and embryonic stem cells.

This product has been discontinued.
Catalogue NumberSC1000
Brand Family Calbiochem®
SynonymsAnti-Nanog Homeobox, Anti-ENK, Anti-Early Embryo Specific Expression NK Family Gene
Application Data
Panel A: Detection of mouse Nanog by immunoblotting. Samples: Whole cell lysate from mouse myleoid cells (15 µg, lane 1) and mouse embryonic stem cells (5 µg, lane 2). Primary antibody: Anti-Nanog Rabbit pAb (Cat. No. SC1000) (0.1 µg/ml). Detection: chemiluminescence. Panel B: Detection of mouse Nanog by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from mouse embryonic stem cells. Antibody for IP: Anti-Nanog Rabbit pAb (Cat. No. SC1000) (1 µg/mg total protein). Immunoblotting conditions: same as panel A.
References
ReferencesChambers, I., et al. 2003. Cell 113, 643.
Mitsui, K., et al. 2003. Cell 113, 631.
Pan, G.J. and Pei, D.Q. 2003. Cell Res. 13, 499.
Product Information
FormLiquid
FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
Positive controlMouse myeloid embryonic stem cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (1:5000-1:15,000)
Immunoprecipitation (1-2 µg/ml lysate)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids within residues 1-50 of mouse Nanog
ImmunogenHuman
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
SC1000 0

Documentation

Anti-Nanog Rabbit pAb Certificates of Analysis

TitleLot Number
SC1000

References

Hivatkozások áttekintése
Chambers, I., et al. 2003. Cell 113, 643.
Mitsui, K., et al. 2003. Cell 113, 631.
Pan, G.J. and Pei, D.Q. 2003. Cell Res. 13, 499.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-October-2007 RFH
SynonymsAnti-Nanog Homeobox, Anti-ENK, Anti-Early Embryo Specific Expression NK Family Gene
ApplicationImmunoblotting (1:5000-1:15,000)
Immunoprecipitation (1-2 µg/ml lysate)
Application Data
Panel A: Detection of mouse Nanog by immunoblotting. Samples: Whole cell lysate from mouse myleoid cells (15 µg, lane 1) and mouse embryonic stem cells (5 µg, lane 2). Primary antibody: Anti-Nanog Rabbit pAb (Cat. No. SC1000) (0.1 µg/ml). Detection: chemiluminescence. Panel B: Detection of mouse Nanog by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from mouse embryonic stem cells. Antibody for IP: Anti-Nanog Rabbit pAb (Cat. No. SC1000) (1 µg/mg total protein). Immunoblotting conditions: same as panel A.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~40 kDa Nanog protein.
BackgroundNanog is a newly identified homeodomain gene product that directs propagation of undifferentiated embryonic stem (ES) cells and was named after the mythological Celtic land of the eternal youth, Tir Na Nog. Nanog is present in both mouse and human embryonic stem cells, and lacking in differentiated cells. It is restricted to founder cells from which ES cells can be derived. Nanog is important for maintaining the self-renewing properties of mouse embryonic stem cells and is expressed in the cells of the early embryo that are used to derive embryonic stem cells. Current research suggests that Nanog acts independently of another signal transduction pathway important to maintaining the pluripotency of stem cells, which involves the activation of Stat3.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids within residues 1-50 of mouse Nanog
IsotypeIgG
Speciesmouse
Positive controlMouse myeloid embryonic stem cells
FormLiquid
FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesChambers, I., et al. 2003. Cell 113, 643.
Mitsui, K., et al. 2003. Cell 113, 631.
Pan, G.J. and Pei, D.Q. 2003. Cell Res. 13, 499.