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ABE81 Anti-NXF1 Antibody

Use Anti-NXF1 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect NXF1 also known as Nuclear RNA export factor 1, Tip-associated protein.

Anti-NXF1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: NXF1, TAP UniProt Number: Q9UBU9
ABE81
100 µL  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWBRbSerumPolyclonal Antibody
Description
Catalogue NumberABE81
DescriptionAnti-NXF1 Antibody
Alternate Names
  • Nuclear RNA export factor 1
  • Tip-associated protein
  • Tip-associating protein
  • mRNA export factor TAP
Background InformationNuclear RNA export factor 1 (also known as Tap protein) is encoded by the NXF1 gene. The protein expressed by this gene is one member of a family of nuclear RNA export factor gene. The Nuclear RNA export factor 1 protein is involved in export of mRNA species bearing retroviral constitutive transport elements (CTE) from the nucleus as well as in the export of mRNA from the nucleus to the cytoplasm. This protein is localized predominantly in the nucleoplasm at both the nucleoplasmic and cytoplasmic faces of the nuclear pore complex. However, this protein shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A) RNA. This protein is present in the nuclear Y14 complex and has been shown to interact with Aly/REF.
References
Product Information
FormatSerum
Control
  • A431 cell lysate
PresentationRabbit polyclonal serum containing 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationUse Anti-NXF1 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect NXF1 also known as Nuclear RNA export factor 1, Tip-associated protein.
Key Applications
  • Western Blotting
Biological Information
ImmunogenRecombinant protein corresponding to human NXF1.
HostRabbit
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq].
Gene Symbol
  • NXF1
  • TAP
Purification MethodUnpurified
UniProt Number
UniProt SummaryFUINCTION: Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with THOC4 and THOC5.

SUBUNIT STRUCTURE: Heterodimer with NXT1. Interacts (via NTF2 domain) with NXT1. The formation of NXF1/NXT1 heterodimers is required for the NXF1-mediated nuclear mRNA export. Interacts with NXT2, E1B-AP5, RAE1, THOC4 and with several nucleoporins. Is part of the exon junction complex (EJC) containing NCBP1, NCBP2, RNPS1, RBM8A, SRRM1, NXF1, RENT1, RENT2, RENT3A, RENT3B, THOC4 and/or REFBP2. Found in a mRNA complex with RENT3A and RENT3B. Interacts with FYTTD1/UIF, EIF4A3, NUPL2 and THOC5. Interacts with Saimiriine herpesvirus 2 TIP protein.

SUBCELLULAR LOCATION: Nucleus › nucleoplasm. Nucleus speckle. Cytoplasm. Note: Localized predominantly in the nucleoplasm and at both the nucleoplasmic and cytoplasmic faces of the nuclear pore complex. Shuttles between the nucleus and the cytoplasm. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA.

TISSUE SPECIFICTY: Expressed ubiquitously.

DOMAIN: The minimal CTE binding domain consists of an RNP-type RNA binding domain (RBD) and leucine-rich repeats.

The nucleoporin binding domain consists of a NTF2 domain (also called NTF2-like domain) and a TAP-C domain (also called UBA-like domain). It has 2 nucleoporin-FG-repeats binding sites (one in the NTF2 and the other in the TAP-C domain) which contribute to nucleoporin association and act synergistically to export cellular mRNAs.

The NTF2 domain is functional only in the presence of NXT1 and is essential for the export of mRNA from the nucleus.

The TAP-C domain mediates direct interactions with nucleoporin-FG-repeats and is necessary and sufficient for localization of NXF1 to the nuclear rim. The conserved loop 594-NWD-596 of the TAP-C domain has a critical role in the interaction with nucleoporins.

The leucine-rich repeats are essential for the export of mRNA from the nucleus.

MISCELLANEOUS: The RNA-binding domain is a non-canonical RNP-type domain.

SEQUENCE SIMILARITIES: Belongs to the NXF family.

Contains 4 LRR (leucine-rich) repeats.

Contains 1 NTF2 domain.

Contains 1 RRM (RNA recognition motif) domain.

Contains 1 TAP-C domain.
Molecular Weight~72 kDa observed. Two isoforms at 70 and 40 kDa have been described. Uncharacterized bands at ~48 and 100 kDa have been observed in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected NXF1 on 10 µg of A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
ABE81 04053252591716