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ABE1456 Anti-NR4A3

Anti-NR4A3, Cat. No. ABE1456, is a highly specific rabbit polyclonal antibody that detects Nuclear receptor subfamily 4 group A member 3 (NR4A3) and has been tested for use in Immunocytochemistry and Western Blotting.

Anti-NR4A3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: NR4A3, CSMF, MINOR, NOR1, TEC UniProt Number: Q92570
ABE1456
100 μg  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE1456
DescriptionAnti-NR4A3
Alternate Names
  • Nuclear receptor subfamily 4 group A member 3
  • Mitogen-induced nuclear orphan receptor
  • Neuron-derived orphan receptor 1
  • Nuclear hormone receptor NOR-1
Background InformationNuclear receptor subfamily 4 group A member 3 (UniProt: Q92570; also known as NR4A3, Mitogen-induced nuclear orphan receptor, Neuron-derived orphan receptor 1, Nuclear hormone receptor NOR-1) is encoded by the NR4A3 (also known as CHN, CSMF, MINOR, NOR1, TEC) gene (Gene ID: 8013) in human. NR4A3 acts as a transcriptional activator that binds to regulatory elements in promoter regions in a cell- and response element (target)-specific manner. It induces gene expression by binding as monomers to the NR4A1 response element (NBRE) 5'-AAAAGGTCA-3' site and as homodimers to the Nur response element (NurRE) site in the promoter of their regulated target genes. Its DNA binding region is located within amino acids 289-364. Isoform alpha of NR4A3 is highly expressed in skeletal muscle and isoform beta is predominantly expressed in skeletal muscle. Lower expression levels have been reported in fetal brain and in placenta. NR4A3 plays a role in the regulation of proliferation, survival and differentiation of many different cell types and also in metabolism and inflammation. It is also shown to negatively regulate myeloid progenitor cell proliferation by repressing RUNX1 in a NBRE site-independent manner. NR4A3 activity is induced by inflammatory stimuli in endothelial cells through an NF-kappa-B-dependent transactivation of the NR4A3 promoter. NR4A3 is reported to modulate feeding behavior and is involved in energy balance by inhibiting the glucocorticoid-induced orexigenic neuropeptides AGRP expression. It also regulates glucose transport by regulating translocation of the SLC2A4 transporter to the cell surface. Mutations in NR4A3 gene have been linked to Ewing sarcoma, a highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-NR4A3, Cat. No. ABE1456, is a highly specific rabbit polyclonal antibody that detects Nuclear receptor subfamily 4 group A member 3 (NR4A3) and has been tested for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected NR4A3 in HeLa, A431, and NIH/3T3 cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 25 amino acids from the N-terminal region of human Nuclear receptor subfamily 4 group A member 3 (NR4A3).
EpitopeN-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Nuclear receptor subfamily 4 group A member 3 (NR4A3) in human and murine cells.
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • NR4A3
  • CSMF
  • MINOR
  • NOR1
  • TEC
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~59 kDa observed; 68.23 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected NR4A3 in 10 µg of HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
ABE1456 04054839416569