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AB10239 Anti-NQO-1 Antibody

Anti-NQO-1 Antibody detects level of NQO-1 & has been published & validated for use in WB.

Research Category: Metabolism Research Sub-Category: Toxicology & Drug Resistance UniProt Number: P15559
AB10239
50 µL  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, R, PmWBRbSerumPolyclonal Antibody
Description
Catalogue NumberAB10239
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-NQO-1 Antibody
Alternate Names
  • NAD(P)H dehydrogenase
  • quinone 1
  • Menadione reductase
  • NAD(P)H menadione oxidoreductase 1, dioxin-inducible
  • NAD(P)H:Quinone acceptor oxidoreductase type 1
  • NAD(P)H:menadione oxidoreductase 1
  • NAD(P)H:quinone oxidoreductase 1
  • NAD(P)H:quinone oxireductase
  • Phylloquinone reductase
  • Quinone reductase 1
  • diaphorase (NADH/NADPH) (cytochrome b-5 reductase)
  • dioxin-inducible 1
Background InformationNAD(P)H dehydrogenase, quinone 1, also known as NQO-1, is a human gene which encodes a cytoplasmic 2-electron reductase. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD).
References
Product Information
FormatSerum
PresentationRaw rabbit serum with 0.05% sodium azide.
Applications
ApplicationAnti-NQO-1 Antibody detects level of NQO-1 & has been published & validated for use in WB.
Key Applications
  • Western Blotting
Application NotesWestern Blot Analysis: A 1:5000 dilution of this lot detected baculovirus expressed recombinant NQO-1 protein.
Biological Information
ImmunogenRecombinant NQO-1
EpitopeG115 – K274
HostRabbit
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Primate
Species Reactivity NoteHuman. Expected to react with mouse, rat and primate based on sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
UniProt Number
UniProt SummaryFunction: The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
Catalytic activity: NAD(P)H + a quinone = NAD(P)(+) + a hydroquinone.
Cofactor: FAD.
Enzyme regulation: Inhibited by dicoumarol.
Subunit structure: Homodimer.
Subcellular location: Cytoplasm.
Induction By: dioxin.
Polymorphism: The Ser-187 polymorphism may be linked to susceptibility to forms of cancers.
Miscellaneous: Quinone reductase accepts electrons from both NADH and NADPH with equal efficiency.
Sequence similarities: Belongs to the NAD(P)H dehydrogenase (quinone) family.
Molecular Weight31 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on baculovirus expressed recombinant NQO-1.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months from date of receipt
Packaging Information
Material Size50 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
AB10239 04053252469893

Documentation