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DR1050 Anti-NFE2L2 Mouse mAb (1F3)

This Anti-NFE2L2 Mouse mAb (1F3) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of NFE2L2.

Anti-NFE2L2 Mouse mAb (1F3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Nuclear Factor (Erythroid-Derived 2) Like 2

DR1050
  
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Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Host
M
Description
Overview

This product has been discontinued.





Recognizes the NFE2L2 protein in human lung tissue and HeLa cells.
Catalogue NumberDR1050
Brand Family Calbiochem®
SynonymsAnti-Nuclear Factor (Erythroid-Derived 2) Like 2
Application Data
Detection of human NFE2L2 by immunoblotting. Sample: Partial recombinant NFE2L2. Primary antibody: Anti-NFE2L2 Mouse mAb (1F3) (Cat. No. DR1050) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human NFE2L2 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human lung tissue. Primary antibody: Anti-NFE2L2 Mouse mAb (1F3) (Cat. No. DR1050) (1 µg/ml). Detection: colorimetric (DAB).

Detection of human NFE2L2 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-NFE2L2 Mouse mAb (1F3) (Cat. No. DR1050) (10 µg/ml). Detection: fluorescence.
References
ReferencesLindl, K,A., and Jordan-Sciutto, K,L., Methods Mol. Biol. 477 229.
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlHuman lung tissue, HeLa cells
PreservativeNone
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Paraffin Sections (1 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids 71-170 of human NFE2L2, expressed as a GST fusion protein
Clone1F3
HostMouse
IsotypeIgG2b
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
DR1050 0

Documentation

Anti-NFE2L2 Mouse mAb (1F3) MSDS

Title

Safety Data Sheet (SDS) 

References

Hivatkozások áttekintése
Lindl, K,A., and Jordan-Sciutto, K,L., Methods Mol. Biol. 477 229.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-November-2010 RFH
SynonymsAnti-Nuclear Factor (Erythroid-Derived 2) Like 2
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Paraffin Sections (1 µg/ml)
Application Data
Detection of human NFE2L2 by immunoblotting. Sample: Partial recombinant NFE2L2. Primary antibody: Anti-NFE2L2 Mouse mAb (1F3) (Cat. No. DR1050) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human NFE2L2 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human lung tissue. Primary antibody: Anti-NFE2L2 Mouse mAb (1F3) (Cat. No. DR1050) (1 µg/ml). Detection: colorimetric (DAB).

Detection of human NFE2L2 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-NFE2L2 Mouse mAb (1F3) (Cat. No. DR1050) (10 µg/ml). Detection: fluorescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the NFE2L2 protein.
BackgroundNFE2, NFE2L1, and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, such as JUN and FOS, although remaining regions have diverged considerably from each other.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids 71-170 of human NFE2L2, expressed as a GST fusion protein
Clone1F3
IsotypeIgG2b
Specieshuman
Positive controlHuman lung tissue, HeLa cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review
ReferencesLindl, K,A., and Jordan-Sciutto, K,L., Methods Mol. Biol. 477 229.