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MABC1235 Anti-N-Myc Interactor Antibody, clone 16E9.2

Detect N-Myc Interactor using this mouse monoclonal Anti-N-Myc Interactor Antibody, clone 16E9.2, Cat. No. MABC1235, validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Anti-N-Myc Interactor Antibody, clone 16E9.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: NMI UniProt Number: Q13287
MABC1235
100 μg  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
HICC, IH(P), WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1235
DescriptionAnti-N-Myc Interactor Antibody, clone 16E9.2
Alternate Names
  • N-myc-interactor
  • N-myc and STAT interactor
  • Nmi
Background InformationN-myc-interactor (UniProt Q13287; also known as N-myc and STAT interactor, Nmi) is encoded by the NMI gene (Gene ID 9111) in human. Nmi is expressed in all adult and fetal tissues except brain and skin and is shown to be more abundant in fetal liver. It interacts with all STATs, except STAT2 and augments STAT-mediated transcription in response to IL-2 and gamma-interferon. Interestingly, Nmi lacks an intrinsic transcriptional activation domain; instead, it enhances the association of CBP/p300 coactivator proteins with STAT1 and STAT5. Higher expression of Nmi has been reported in myeloid leukemia that also express higher levels of c-Myc. Ref.: Zhu, M., et al. (1999). Cell 96, 121-130.
References
Product Information
FormatPurified
PresentationPurified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect N-Myc Interactor using this mouse monoclonal Anti-N-Myc Interactor Antibody, clone 16E9.2, Cat. No. MABC1235, validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected N-Myc Interactor in 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized HeLa and A431 cells.

Immunocytochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected N-Myc Interactor in human cerebellum, small instestine, and tonsil tissue sections.
Biological Information
ImmunogenGST-tagged recombinant human N-Myc Interactor N-terminal fragment.
Clone16E9.2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 16E9.2 targeted epitope resides within the N-terminal half of N-Myc Interactor.
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • NMI
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~39 kDa observed. 35.06 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected N-Myc Interactor in 10 µg of K562 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABC1235 04054839105470