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MABT838-25UG Anti-Myosin 7 (MYH7) Antibody, clone BA-D5

Anti-Myosin 7 (MYH7), clone BA-D5, Cat. No. MABT838, is a mouse monoclonal antibody that detects Myosin-7 and has been tested for use in Immunofluorescence, Immunohistochemistry, and Western Blotting.

MABT838-25UG
25 μg  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue NumberMABT838-25UG
DescriptionAnti-Myosin 7 (MYH7) Antibody, clone BA-D5
Alternate Names
  • Myosin heavy chain 7
  • Myosin heavy chain slow isoform
  • MyHC-slow
  • Myosin heavy chain
  • Cardiac muscle beta isoform
  • MyHC-beta)
Background InformationMyosin-7 (UniProt: Q9BE39; also known as Myosin heavy chain 7, Myosin heavy chain slow isoform, MyHC-slow, Myosin heavy chain, cardiac muscle beta isoform, MyHC-beta) is encoded by the MYH7 gene (Gene ID: 282714) in bovine species. Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Myosins form regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle. Myosin-7 is a component of thick filaments of the myofibrils. It contains actin- and ATP-binding sites in its conserved catalytic head domain. Its actin binding region is localized to amino acids 655-677 and 757-771 and the ATP-binding region is localized to amino acids 178-185. Myosin-7 has an N-terminal SH3-like domain (aa 32-81), a myosin motor domain (aa 85-778), and an IQ domain (aa 781-810). Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Limited proteolysis of myosin heavy chain produces one light meromyosin (LMM) and one heavy meromyosin (HMM). HMM can be further cleaved into two globular sub fragments (S1) and 1 rod-shaped sub fragment (S2).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Myosin 7 (MYH7), clone BA-D5, Cat. No. MABT838, is a mouse monoclonal antibody that detects Myosin-7 and has been tested for use in Immunofluorescence, Immunohistochemistry, and Western Blotting.
Key Applications
  • Immunofluorescence
  • Immunohistochemistry
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A representative lot detected Myosin 7 (MYH7) in Immunohistochemistry applications (Goh, Q., et. al. (2017). Elife. 6. pii: e20007).

Western Blotting Analysis: A representative lot detected Myosin 7 (MYH7) in Western Blotting applications (De Jong, A., et. al. (2017). PLoS One. 12(3):e0174043; Gan, Z., et. al. (2013). J Clin Invest. 123(6):2564-75).

Immunofluorescence Analysis: A representative lot detected Myosin 7 (MYH7) in Immunofluorescence applications (Gan, Z., et. al. (2013). J Clin Invest. 123(6):2564-75; Pannerec, A., et. al. (2016). Aging (Albany NY). 8(4):712-29).
Biological Information
ImmunogenPurified myosin from bovine atrium.
CloneBA-D5
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone BA-D5 detects Myosin-7 in multiple mammalian species. It detects both alpha- and beta-slow myosin heavy chain.
IsotypeIgG2bκ
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteMouse, Human.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Myh7
Purification MethodProtein G purified
UniProt Number
Molecular Weight~220 kDa observed; 223.23 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse soleus muscle tissue lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected Myosin 7 (MYH7) in mouse soleus muscle tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABT838-25UG 04054839500329

Documentation

Anti-Myosin 7 (MYH7) Antibody, clone BA-D5 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Myosin 7 (MYH7) Antibody, clone BA-D5 Certificates of Analysis

TitleLot Number
Anti-Myosin 7 (MYH7), clone BA-D5 - 3890571 3890571
Anti-Myosin 7 (MYH7), clone BA-D5 - 3916240 3916240
Anti-Myosin 7 (MYH7), clone BA-D5 - 3988541 3988541
Anti-Myosin 7 (MYH7), clone BA-D5 - 4029344 4029344
Anti-Myosin 7 (MYH7), clone BA-D5 - 4159419 4159419
Anti-Myosin 7 (MYH7), clone BA-D5 - 4205463 4205463
Anti-Myosin 7 (MYH7), clone BA-D5 Monoclonal Antibody Q3041338

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