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MABF2074-25UL Anti-MuLV TM Antibody, clone 42-114

Anti-MuLV TM, clone 42-114, Cat. No. MABF2074, is a rat monoclonal antibody that detects Murine leukemia virus (MuLV) transmembrane protein and has been tested for use in Immunoprecipitation and Western Blotting.

Anti-MuLV TM Antibody, clone 42-114 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology
MABF2074-25UL
25 µL  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
VirusIP, WBRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF2074-25UL
DescriptionAnti-MuLV TM Antibody, clone 42-114
Alternate Names
  • Murine leukemia virus
Background InformationThe murine leukemia virus (MuLV) is a gammaretrovirus that has a positive, single-stranded sense RNA, which replicates via reverse transcription. The envelope of the virus is shown to be covered with glycoprotein spikes. Three major genes produced by the murine leukemia virus are gag, pol, and env. The gag gene codes for the group-specific antigen that is responsible for the production of the viral matrix capsid and nucleoproteins. The pol gene encodes reverse transcriptase, a protease, and an integrase. The reverse transcriptase is used to make complementary DNA by reverse transcribing its own RNA into DNA. The reverse transcriptase in MuLV is able to act as a monomer as opposed to a dimer. The integrase integrates the synthesized viral DNA into the host cell s DNA. The env gene codes for a glycosylated protein that is processed into the two viral envelope proteins, gp70 and p15(E), which aid the virus in selecting and attacking host cells. The gp70 protein has the receptor binding activity of the virus. Retrovirus membrane fusion is catalyzed by the Env, surface (SU) and transmembrane (TM) proteins. The SU protein is involved in receptor binding, while the TM protein contains the necessary elements for membrane fusion. Clone 42-114 is TM-specific and does not recognize G541R mutants. (Ref. Pinter,A., et al. (1982). Virology, 116(2); 499-516. Schneider, WM., et al. (2008). Virology. 371(1); 165-174).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgM in PBS with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-MuLV TM, clone 42-114, Cat. No. MABF2074, is a rat monoclonal antibody that detects Murine leukemia virus (MuLV) transmembrane protein and has been tested for use in Immunoprecipitation and Western Blotting.
Key Applications
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated MuLV TM (Pinter, A., et. al. (1982). Virology. 116(2):499-516).

Western Blotting Analysis: A representative lot detected MuLV TM in Western Blotting applications (Schneider, W.M., et. al. (2008). Virology. 371(1):165-74).
Biological Information
ImmunogenAKR K36 and AKR SL3 leukemia virus.
Clone42-114
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone 42-114 detects murine leukemia virus in infected cells.
IsotypeIgMκ
Species Reactivity
  • Virus
Species Reactivity NoteMurine Leukemia Virus.
Antibody TypeMonoclonal Antibody
Molecular Weight~15 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cells infected with murine leukemia virus (MuLVs).

Western Blotting Analysis: A 1:250 dilution of this antibody detected MuLV TM in HEK293 cells infected with murine leukemia virus (MuLVs).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABF2074-25UL 04054839448263