Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

09-872-I-100UG Anti-Methylcytosine dioxygenase TET1

Anti-Methylcytosine dioxygenase TET1, Cat. No. 09-872-I is a rabbit polyclonal antibody that detects Methylcytosine dioxygenase TET1 and is tested for use in Western Blotting.

09-872-I-100UG
100 μg  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue Number09-872-I-100UG
DescriptionAnti-Methylcytosine dioxygenase TET1
Alternate Names
  • Methylcytosine dioxygenase TET1
  • CXXC-type zinc finger protein 6
  • Ten-eleven translocation 1 gene protein homolog
Background InformationMethylcytosine dioxygenase TET1 (UniProt: Q3URK3; also known as CXXC-type zinc finger protein 6, Ten-eleven translocation 1 gene protein homolog) is encoded by the Tet1 (also known as Cxxc6, Kiaa1676) gene (Gene ID: 52463) in murine species. TET1 is a dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. It is shown to be present in embryonic stem cells. Presence of 5hmC has been reported in the brain, especially in Purkinje neurons. In addition to its role in DNA demethylation, TET1 also plays a more general role in chromatin regulation. It preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and Polycomb-repressed genes. It is involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. TET family consists of three TET enzymes that contain cysteine rich and DSBH domains. TET1 and TET3 also have a CXXC domain, which typically bind unmethylated CpG dinucleotides, but in TET enzymes they bind 5mC and 5hmC.
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Methylcytosine dioxygenase TET1, Cat. No. 09-872-I is a rabbit polyclonal antibody that detects Methylcytosine dioxygenase TET1 and is tested for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptides corresponding to 12, 14, and 16 amino acids from N-terminal, internal, and C-terminal regions of mouse Methylcytosine dioxygenase TET1.
Epitopeunknown
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects murine Methylcytosine dioxygenase TET1.
IsotypeIgG
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Tet1
  • Cxxc6
  • Kiaa1676
Purification MethodProtein A purified
UniProt Number
Molecular Weight~ 220 kDa observed; 219.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Mouse Thymus tissue lysates.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Methylcytosine dioxygenase TET1 in Mouse Thymus tissue lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
09-872-I-100UG 04061842378241

Documentation

Anti-Methylcytosine dioxygenase TET1 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Methylcytosine dioxygenase TET1 Certificates of Analysis

TitleLot Number
Anti-Methylcytosine dioxygenase TET1 - 3772306 3772306
Anti-Methylcytosine dioxygenase TET1 - 3808750 3808750
Anti-Methylcytosine dioxygenase TET1 - 3837035 3837035
Anti-Methylcytosine dioxygenase TET1 - 3862787 3862787
Anti-Methylcytosine dioxygenase TET1 - 3866911 3866911
Anti-Methylcytosine dioxygenase TET1 - 3900882 3900882
Anti-Methylcytosine dioxygenase TET1 - 3905443 3905443
Anti-Methylcytosine dioxygenase TET1 - Q3477085 Q3477085