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MAB4334Z Sigma-AldrichAnti-MDR1 Antibody, clone UIC2, azide free

Detect MDR1 using this Anti-MDR1 Antibody, clone UIC2, azide free validated for use in FC, IF, IP, IH.

Anti-MDR1 Antibody, clone UIC2, azide free MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA

Research Category: Metabolism Research Sub-Category: Toxicology & Drug Resistance Gene Symbol: ABCB1, PGY1, MGC163296, CD243, GP170, P-gp, MDR1, CLCS, ABC20, EC 3.6.3.44 UniProt Number: P08183

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Kulcsspecifikációk táblázata

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, Pm	FC, IF, IP, IHC, FUNC	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MAB4334Z
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-MDR1 Antibody, clone UIC2, azide free
Alternate Names	P-glycoprotein CD243 p170 Pgp MAB4334
Background Information	UIC2 is a mouse monoclonal antibody directed against the extracellular conformational epitope of P-glycoprotein (Pgp). This antibody can be used in flow cytometry, immunofluorescence microscopy and immunohistochemistry in frozen and paraffin sections to detect Pgp expression on the membrane of MDR1 positive cells. In functional experiments, UIC2 was shown to inhibit Pgp-mediated efflux activity and potentiate the cytotoxic effects of chemotherapy drugs transported by Pgp. Additionally, the UIC2 monoclonal antibody can be used in combination with MDR1 substrates for simulatenaeous detection of Pgp expression and function by flow cytometry (MILLIPORE's UIC2 Shift assay - see product ECM905).

References

Product Information
Format	Purified
Presentation	Purified immunoglobulin from Protein A Sepharose™ chromatography. Sterile filtered in 0.01 M PBS, no preservative.
Quality Level	MQ300

Applications
Application	Detect MDR1 using this Anti-MDR1 Antibody, clone UIC2, azide free validated for use in FC, IF, IP, IH.
Key Applications	Flow Cytometry Immunofluorescence Immunoprecipitation Immunohistochemistry Affects Function
Application Notes	Inhibition of P-glycoprotein efflux activity in functional experiments: 10 - 100 μg/mL, varies in different systems Immunoprecipitation: 5 - 10 fold molar excess of UIC2 over the expected Pgp concentration; realistically, 1 - 5 ug per 1 μl of cell lysate Flow Cytometry (conventional and UIC2 Shift assay): depends highly on Pgp expression by target cells; usually, 0.1 μg/mL for low Pgp expressors and 1 μg/mL for high Pgp expressors Immunofluorescence: depends highly on Pgp expression by target cells; usually, 0.1 μg/mL for low Pgp expressors and 1 μg/mL for high Pgp expressors IHC on frozen and paraffin sections: 1 - 10 μg/mL

Biological Information
Immunogen	Mouse BALB/c 3T3 fibroblasts transfected with human MDR1 cDNA, followed by a multistep selection of tranfectants for resistance to vinblastine.
Epitope	conformational extracellular epitope, azide free
Clone	UIC2
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	P-glycoprotein encoded by the MDR1 gene (ABCB1; LocusLink ID: 5243)
Isotype	IgG2aκ
Species Reactivity	Human Primate
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_000927.3
Entrez Gene Summary	The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.
Gene Symbol	ABCB1 PGY1 MGC163296 CD243 GP170 P-gp MDR1 CLCS ABC20 EC 3.6.3.44
Non-Reactive Species	Mouse Rat
UniProt Number	P08183
UniProt Summary	FUNCTION: SwissProt: P08183 # Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. SIZE: 1280 amino acids; 141463 Da SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed in liver, kidney, small intestine and brain. SIMILARITY: SwissProt: P08183 ## Belongs to the ABC transporter family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. & Contains 2 ABC transmembrane type-1 domains. & Contains 2 ABC transporter domains.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain at 2-8°C for up 12 months.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Katalógusszám	GTIN
MAB4334Z	04053252723605

Documentation

Anti-MDR1 Antibody, clone UIC2, azide free MSDS

Title
Safety Data Sheet (SDS)

Anti-MDR1 Antibody, clone UIC2, azide free Certificates of Analysis

Title	Lot Number
Mouse Monoclonal Anti-UIC2 (MDR1 P-glycoprotein) SODIUM AZIDE FREE Mouse Monoclonal Antibody - 2379332	2379332
Mouse Monoclonal Anti-UIC2 (MDR1 P-glycoprotein) - 3725267	3725267
Mouse Monoclonal Anti-UIC2 (MDR1 P-glycoprotein) -2524062	2524062
Mouse Monoclonal Anti-UIC2 (MDR1 P-glycoprotein) -2624591	2624591
Mouse Monoclonal Anti-UIC2 (MDR1 P-glycoprotein) -2805164	2805164
Mouse Monoclonal Anti-UIC2 (MDR1 P-glycoprotein) -2810621	2810621
Mouse Monoclonal Anti-UIC2 (MDR1 P-glycoprotein) SODIUM AZIDE FREE - 3321109	3321109
Mouse Monoclonal Anti-UIC2 (MDR1 P-glycoprotein) SODIUM AZIDE FREE - 3702343	3702343
Mouse Monoclonal Anti-UIC2 (MDR1 P-glycoprotein) SODIUM AZIDE FREE Mouse Monoclonal Antibody	2879769
Mouse Monoclonal Anti-UIC2 (MDR1 P-glycoprotein) SODIUM AZIDE FREE Mouse Monoclonal Antibody	2891001

Kapcsolódó termékek és alkalmazások

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MAB4334	Anti-MDR1 Antibody, conformational extracellular epitope, clone UIC2

Kategóriák

Life Science Research > Antibodies and Assays > Primary Antibodies

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