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MAB16985B Anti-MCAM Antibody, clone P1H12, biotin conjugated

This Anti-MCAM Antibody, clone P1H12, biotin conjugated is validated for use in ELISA, FC, IP, IC, IH for the detection of MCAM.

Anti-MCAM Antibody, clone P1H12, biotin conjugated MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: MCAM, Gicerin, MUC18, CD146 UniProt Number: P43121
MAB16985B
100 µg  
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Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
Ca, H, MELISA, FC, IP, ICC, IHCMBiotinMonoclonal Antibody
Description
Catalogue NumberMAB16985B
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-MCAM Antibody, clone P1H12, biotin conjugated
Alternate Names
  • MUC18
  • CD146
Background InformationMUC18 antigen (CD146), a member of the immuoglobulin superfamily, is an intrinsic membrane glycoprotein of 110-120 kDa found on the surface of endothelial cells, bone marrow fibroblasts and various melanomas. MUC18 has been used as a marker of tumor progression in human melanoma because expression in those tumors correlates strongly with poor prognosis and the development of metastic disease. In addition, a number of human T, B and myeloid leukemic cell lines seem to express MUC18. The close structural relationship with N-CAM and related molecules suggests that MUC18 may be also a developmentally regulated cell adhesion molecule (Melanoma adhesion molecule or MCAM).
References
Product Information
FormatBiotin
PresentationPurified immunoglobulin. Liquid in 0.02M PBS, 0.25M NaCl, pH=7.6, 15 mg/nL BSA containing 0.1% sodium azide as a preservative.
Quality LevelMQ100
Applications
ApplicationThis Anti-MCAM Antibody, clone P1H12, biotin conjugated is validated for use in ELISA, FC, IP, IC, IH for the detection of MCAM.
Key Applications
  • ELISA
  • Flow Cytometry
  • Immunoprecipitation
  • Immunocytochemistry
  • Immunohistochemistry
Application NotesImmunocytochemistry: 1-10 μg/mL. Works best on EDTA or Trypsin lifted endothelial cells.

Immunohistochemistry: 1-10 μg/mL. 4% PFA for 30min RT or <2hrs @4°C. Block w/ 1%BSA/0.2%tween20/PBC for 30min. Works well in frozen tissue; fixed or unfixed.

Immunoprecipitation: 1-10 μg/mL

ELISA: 1-10 μg/mL

Facs Analysis: 1-10 μg/mL

Optimal working dilutions and protocols must be determined by end user.
Biological Information
ImmunogenImmunization of mice with HUVEC
CloneP1H12
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityP1H12 reacts specifically with CD146 {MCAM, MUC18}. In blood and marrow P1H12 reacts only with endothelial cells, and has been used to detect circulating endothelial cells in human, dogs and mouse samples. It positively stains normal, primary HUVEC and MVEC cultures and the endothelial cells of all vessels in normal frozen sections of human skin, intestine, ovary tonsil, lymph node, lung, and kidney. It does not stain carcinoma cell lines HT-29 and COLO205, and M21, the T cell lines Jurkat and HuT78, fibroblasts, HL-60 or CHO cells, or EBV-transformed B cell lines, although it expected that CD146 expression is present in many tumor lines. P1H12 does not stain normal monocytes, granulocytes, red cells, platelets, T cells or B cells from marrow or peripheral blood; nor does it detect marrow megakaryocytes or the megakaryoblast line HU3. The peripheral blood cells that do stain with P1H12 also are positive for both von Willebrand Factor (vWF) and thrombomodulin (the combined expression of which is limited to endothelium), and they stain for flt and flk. In recent testing, melanoma A2058, SKMEL.3 and A375.S2 are positive, while murine melanoma M3 is negative. One smooth muscle line (HISM) was positive and one negative (TIGHA-VSMC). Human blood lymphoctes are negative with or without prior stimulation.
IsotypeIgG1
Species Reactivity
  • Canine
  • Human
  • Mouse
Species Reactivity NoteDetects circulating endothelial cells in human. Expected to cross-react with dog & mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MCAM
  • Gicerin
  • MUC18
  • CD146
Non-Reactive Species
  • Rat
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P43121 # Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.
SIZE: 646 amino acids; 71607 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.
SIMILARITY: SwissProt: P43121 ## Contains 3 Ig-like C2-type (immunoglobulin-like) domains. & Contains 2 Ig-like V-type (immunoglobulin-like) domains.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at 2-8°C for up to 12 months.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MAB16985B 04053252727689

Documentation

Anti-MCAM Antibody, clone P1H12, biotin conjugated MSDS

Title

Safety Data Sheet (SDS) 

Anti-MCAM Antibody, clone P1H12, biotin conjugated Certificates of Analysis

TitleLot Number
MOUSE ANTI-ENDOTHELIAL CELLS (CD146) 2484201
MOUSE ANTI-ENDOTHELIAL CELLS (CD146) BIOTINYLATED MONOCLONAL ANTIBODY - 2433395 2433395
MOUSE ANTI-ENDOTHELIAL CELLS (CD146) -2621489 2621489
MOUSE ANTI-ENDOTHELIAL CELLS (CD146) -2716229 2716229
MOUSE ANTI-ENDOTHELIAL CELLS (CD146) -2768443 2768443
MOUSE ANTI-ENDOTHELIAL CELLS (CD146) BIOTINYLATED - 3272853 3272853
MOUSE ANTI-ENDOTHELIAL CELLS (CD146) BIOTINYLATED - 3484996 3484996
MOUSE ANTI-ENDOTHELIAL CELLS (CD146) BIOTINYLATED - 3911857 3911857
MOUSE ANTI-ENDOTHELIAL CELLS (CD146) BIOTINYLATED - 3948902 3948902
MOUSE ANTI-ENDOTHELIAL CELLS (CD146) BIOTINYLATED - 4019793 4019793
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References

Reference overviewPub Med ID
Genes expressed in human tumor endothelium.
St Croix, B, et al.
Science, 289: 1197-202 (2000)  1999

Kivonat megmutatása 		10947988 10947988

Data Sheet

Title
MOUSE ANTI-ENDOTHELIAL CELLS (CD146) BIOTINYLATED

Kapcsolódó termékek és alkalmazások

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Alternative Format

Katalógusszám Leírás
MAB16985 Anti-MCAM Antibody, clone P1H12
MAB16985F Anti-MCAM Antibody, clone P1H12, FITC conjugated

Product Families

Endothelial Cell Markers

The thin layer of cells that lines the interior surface of blood vessels and lymphatic vessels, forming an interface between circulating blood or lymph in the lumen and the rest of the vessel wall.
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Kategóriák

Life Science Research > Antibodies and Assays > Primary Antibodies