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442711 Anti-MARCKS Mouse mAb (0.T.89)

442711
  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~68-90 kDa human MARCKS in bovine brain extract.
Catalogue Number442711
Brand Family Calbiochem®
References
ReferencesLi, J., et al. 2005.J. Biol. Chem. 9, 8351.
Lange, A., et al. 1997.J. Biol. Chem. 272, 27345.
Product Information
FormLiquid
FormulationAscites diluted with 40% glycerol.
Positive controlBovine brain
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (1:250-1:500)
Immunoprecipitation (assay dependent)
Application CommentsThe optimal dilution for immunoprecipitation is assay-dependent and must be determined by the end-user.
Biological Information
Immunogenhuman recombinant MARCKS
ImmunogenHuman
EpitopeWithin the C-terminus
Clone0.T.89
HostMouse
IsotypeIgG₁
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). For short-term storage, aliquots may be stored at 4°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
442711 0

Documentation

References

Hivatkozások áttekintése
Li, J., et al. 2005.J. Biol. Chem. 9, 8351.
Lange, A., et al. 1997.J. Biol. Chem. 272, 27345.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-January-2009 JSW
ApplicationImmunoblotting (1:250-1:500)
Immunoprecipitation (assay dependent)
DescriptionMouse monoclonal antibody supplied as diluted ascites. Recognizes the ~68-90 kDa MARCKS protein.
BackgroundA protein kinase C (PKC) substrate and indicator of PKC activation. Upon stimulation PKC phosphorylates MARCKS causing the translocation of MARCKS from the plasma membrane to the cytoplasm. MARCKS is ubiqutously expressed in all tissues and phosphorylated by most isoforms of PKC.
HostMouse
Immunogen speciesHuman
Immunogenhuman recombinant MARCKS
EpitopeWithin the C-terminus
Clone0.T.89
IsotypeIgG₁
Speciesbovine, human
Positive controlBovine brain
FormLiquid
FormulationAscites diluted with 40% glycerol.
Preservative≤0.1% sodium azide
CommentsThe optimal dilution for immunoprecipitation is assay-dependent and must be determined by the end-user.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). For short-term storage, aliquots may be stored at 4°C.
Toxicity Standard Handling
ReferencesLi, J., et al. 2005.J. Biol. Chem. 9, 8351.
Lange, A., et al. 1997.J. Biol. Chem. 272, 27345.