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ST1641 Anti-MAP4K4 Mouse mAb (4A5)

ST1641
Purchase on Sigma-Aldrich

Áttekintés

Kulcsspecifikációk táblázata

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

KatalógusszámCsomagolás Menny./csomag
ST1641-100UG 100 μg
Description
OverviewRecognizes the ~140 kDa MAP4K4 protein in K-562 cells and human breast cancer tissue.
Catalogue NumberST1641
Brand Family Calbiochem®
SynonymsAnti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4
Application Data
Detection of human MAP4K4 by immunoblotitng. Sample: Whole cell lysate from K-562 cells. Primary antibody: Anti-MAP4K4 Mouse mAb (4A5) (Cat. No. ST1641) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human MAP4K4 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human breast cancer tissue. Primary antibody: Anti-MAP4K4 Mouse mAb (4A5) (Cat. No. ST1641) (3 µg/ml). Detection: colorimetric (DAB).
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlK-562 cells, Human breast cancer tissue
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (3 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids of 611-710 human MAP4K4, expressed as a GST fusion protein
Clone4A5
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Global Trade Item Number
Katalógusszám GTIN
ST1641-100UG 04055977223774