Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABS813 Anti-MAP2K2, clone 7F5 Antibody

Anti-MAP2K2, clone 7F5 Antibody is an antibody against MAP2K2 for use in Western Blotting, Immunocytochemistry, Flow Cytometry.

Anti-MAP2K2, clone 7F5 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: MAP2K2, MEK2, MKK2, PRKMK2 UniProt Number: P36507
MABS813
100 µL  
Purchase on Sigma-Aldrich

Különleges ajánlatok

Áttekintés

Replacement Information

Különleges ajánlatok

100% Performance Guaranteed

Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, ICC, FCMAscitesMonoclonal Antibody
Description
Catalogue NumberMABS813
Replaces04-377
DescriptionAnti-MAP2K2, clone 7F5 Antibody
Alternate Names
  • Dual specificity mitogen-activated protein kinase kinase 2
  • MAP kinase kinase 2
  • MAPKK 2
  • MAP2K2
Background InformationDual specificity mitogen-activated protein kinase kinase 2 (MAP2K2), also called MEK2, is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax.
References
Product Information
FormatAscites
PresentationMouse monoclonal IgG1ascitic fluid containing 0.03% sodium azide.
Quality LevelMQ300
Applications
ApplicationAnti-MAP2K2, clone 7F5 Antibody is an antibody against MAP2K2 for use in Western Blotting, Immunocytochemistry, Flow Cytometry.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Flow Cytometry
Application NotesImmunocytochemistry Analysis (ICC): A 1:200-1,000 dilution from a representative lot detected MAP2K2 in HeLa cells.

Flow Cytometry Analysis (FC): A 1:200-400 dilution from a representative lot detected MAP2K2 in HeLa cells.
Biological Information
ImmunogenPurified recombinant fragment of human MAP2K2 expressed in E. Coli.
Clone7F5
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Gene Symbol
  • MAP2K2
  • MEK2
  • MKK2
  • PRKMK2
Purification MethodUnpurified
UniProt Number
Molecular Weight~48 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in PC-12, Jurkat, HeLa and NIH/3T3 cell lysates.

Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected MAP2K2 in PC-12, Jurkat, HeLa and NIH/3T3 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABS813 04055977172317