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MABS2001 Anti-LRP-1 Antibody, clone 11H4

Anti-LRP-1, clone 11H4, Cat. No. MABS2001, is a mouse monoclonal antibody that detects Prolow-density lipoprotein receptor-related protein 1 in human, mouse, and rat and has been tested for use in Immunohistochemistry (Paraffin), Immunocytochemistry, Western Blotting and Immunoprecipitation.

Anti-LRP-1 Antibody, clone 11H4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: LRP1, A2MR, APR UniProt Number: Q07954
MABS2001
100 μg  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, MIH(P), ICC, WB, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS2001
DescriptionAnti-LRP-1 Antibody, clone 11H4
Alternate Names
  • Prolow-density lipoprotein receptor-related protein 1
  • Alpha-2-macroglobulin receptor1
  • A2MR
  • Apolipoprotein E receptor
  • APOER
  • CD91
Background InformationProlow-density lipoprotein receptor-related protein 1 (UniProt: Q07954; also known as LRP-1, Alpha-2-macroglobulin receptor1, A2MR, Apolipoprotein E receptor, APOER, CD91) is encoded by the LRP1 (also known as A2MR, APR) gene (Gene ID: 4035) in human. LRP-1 is expressed in most tissues, but is abundant in liver, brain, and lung. LRP1 is synthesized with a signal peptide sequence (a.a. 1-19) and is processed in trans-Golgi network by furin to generate a 515 kDa alpha subunit and an 85 kDa beta subunit. The alpha and beta subunits are non-covalently linked during LRP1 transport to the cell membrane. LRP-1 recognizes and mediates the endocytosis of more than 40 different ligands, including apolipoprotein E (ApoE), APP and amyloid beta. It is the primary receptor mediating transport of amyloid beta peptides across the blood-brain barrier into circulation, thereby clearing them from the brain. LRP-1 is required for early embryonic development and is involved in cellular lipid homeostasis and plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin). Excessive copper accumulation in the brain has been linked with reduced LRP-1 mediated clearance of amyloid beta peptides across the blood brain barrier, which may contribute to complications of Alzheimer's disease.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-LRP-1, clone 11H4, Cat. No. MABS2001, is a mouse monoclonal antibody that detects Prolow-density lipoprotein receptor-related protein 1 in human, mouse, and rat and has been tested for use in Immunohistochemistry (Paraffin), Immunocytochemistry, Western Blotting and Immunoprecipitation.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Immunocytochemistry
  • Western Blotting
  • Immunoprecipitation
Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected LRP-1 in human liver tissue.

Immunoprecipitation Analysis: A representative lot detected LRP-1 in Immunoprecipitation applications (Nieves, E.C., et. al. (2010). J Biol Chem. 285(17):12595-603).

Western Blotting Analysis: A representative lot detected LRP-1 in Western Blotting applications (Spoelgen, R., et. al. (2009). Neuroscience. 158(4):1460-8; Lleó, A., et. al. (2005). J Biol Chem. 280(29):27303-9; Campana, W.M., et. al. (2006). J Nuerosci. 26(43):11197-207).

Immunocytochemistry Analysis: A representative lot detected LRP-1 in Immunocytochemistry applications (Lleó, A., et. al. (2005). J Biol Chem. 280(29):27303-9).

Immunohistochemistry Analysis: A representative lot detected LRP-1 in Immunohistochemistry applications (Campana, W.M., et. al. (2006). J Nuerosci. 26(43):11197-207).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 13 amino acid from the C-terminal region on human LRP-1.
Clone11H4
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 11H4 detects LRP-1 protein in human, mouse, and rat tissues. It targets an epitope within 13 amino acids from the C-terminal region.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Rat
  • Mouse
Species Reactivity NoteHuman, Mouse, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • LRP1
  • A2MR
  • APR
Purification MethodProtein G purified
UniProt Number
Molecular Weight504.61 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human brain tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected LRP-1 in human brain tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABS2001 04054839367212