Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABN2709-25UG Anti-LRAT Antibody, clone 49569

This mouse monoclonal Anti-LRAT, clone 49569, Cat. No. MABN2709 is tested for use in Western Blotting, Immunohistochemistry and Immunofluorescence, for the detection of Lecithin retinol acyltransferase (LRAT).

ÚjMABN2709-25UG
25 μg  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue NumberMABN2709-25UG
DescriptionAnti-LRAT Antibody, clone 49569
Alternate Names
  • retinyl ester synthase
  • lecithin retinol acyl transferase
Background InformationLecithin retinol acyltransferase (UniProt: Q9JI60) (also known as EC:2.3.1.135) is encoded by the Lrat gene (Gene ID:79235) in mouse. Lecithin-retinol acyltransferase (LRAT) is an essential enzyme involved in the metabolism of retinoids, particularly vitamin A. LRAT contains a conserved domain crucial for its enzymatic activity. The primary function of LRAT is to catalyze the esterification of retinol to produce retinyl esters, a process vital for the storage and transport of vitamin A within the body. LRAT's plays a role in the visual cycle as it contributes to the regeneration of visual pigments in the retina. The expression of LRAT is regulated by dietary vitamin A levels, and it is found in various tissues, including the liver, lungs, testis, and the eye. LRAT is primarily localized to the endoplasmic reticulum. Mutations in the LRAT gene have been linked to a form of retinitis pigmentosa, an inherited retinal disease associated with progressive vision loss.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationThis mouse monoclonal Anti-LRAT, clone 49569, Cat. No. MABN2709 is tested for use in Western Blotting, Immunohistochemistry and Immunofluorescence, for the detection of Lecithin retinol acyltransferase (LRAT).
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunofluorescence
Application NotesTested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LRAT in Retinal pigment epithelium (RPE) microsome lysate.

Immunofluorescence Analysis: A representative lot detected LRAT in Immunofluorescence applications (Batten, M.L., et al. (2004). J. Biol. Chem. 279(11):10422-32; Moise, A.R., et al. (2007). J Biol Chem. 282(3):2081-90).

Immunohistochemistry Analysis: A representative lot detected LRAT in Immunohistochemistry applications (Batten, M.L., et al. (2004). J. Biol. Chem. 279(11):10422-32; Moise, A.R., et al. (2007). J Biol Chem. 282(3):2081-90).

Western Blotting Analysis: A representative lot detected LRAT in Western Blotting applications (Batten, M.L., et al. (2004). J Biol Chem. 279(11):10422-32; Moise, A.R., et al. (2007). J. Biol. Chem. 282(3):2081-90).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenHis-tagged recombinant mouse LRAT fragment corresponding to 91 amino acids from the cytoplasmic domain of mouse LRAT.
EpitopeCytoplasmic domain
Clone49569
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 49569 is a mouse monoclonal antibody that detects LRAT. It targets an epitope within 91 amino acids from the cytoplasmic domain of the protein.
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Lrat
Purification MethodProtein G purified
UniProt Number
Molecular WeightTarget molecular weight ~24 kDa observed. 25.82 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse retina lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LRAT in mouse retina tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABN2709-25UG 04065271270190