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MABN2619-25UL Anti-Kininogen-1 Antibody, cleaved form Antibody, clone 4B12

Anti-Kininogen-1, cleaved form, clone 4B12, Cat. No. MABN2619, is a mouse monoclonal antibody that detects Kininogen-1 and is tested for use in ELISA, Immunohistochemistry (Paraffin), and Immunoprecipitation.

MABN2619-25UL
25 µL  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue NumberMABN2619-25UL
DescriptionAnti-Kininogen-1 Antibody, cleaved form Antibody, clone 4B12
Alternate Names
  • Alpha-2-thiol proteinase inhibitor
  • Fitzgerald factor
  • High molecular weight kininogen
  • HMWK
  • Williams-Fitzgerald-Flaujeac factor
Background InformationKininogen-1 (UniProt: P01042; also known as Alpha-2-thiol proteinase inhibitor, Fitzgerald factor, High molecular weight kininogen, HMWK, Williams-Fitzgerald-Flaujeac factor, KNG) is encoded by the KNG1 (also known as BDK, KNG) gene (Gene ID: 3827) in human. Kininogen (KNG) is the precursor protein of the kallikrein-kinin system. KNGs are shown to act as inhibitors of proteases. Kininigen-1 is a secreted protein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to generate the mature form that can be further cleave into Kininigen-1 heavy chain (HMW; aa 19-380), Kininien-1 light chain (LMW; aa 390-644), T-kinin (aa 376-389), Bradykinin (aa 381-389), lysyl-bradykinin (aa 380-389), and Low molecular weight growth-promoting factor (aa 431-434). Kininogen-1 is reported to exert anti-angiogenic properties and block the proliferation of endothelial cells. It has been identified as a serum biomarker for advanced colorectal adenoma and colorectal cancer. The HMW-kininogen plays a role in blood coagulation by helping to position prekallikrein and factor XI next to factor XII. It is shown to inhibit the thrombin- and plasmin-induced aggregation of thrombocytes. Activation of factor XII triggers HMW-kininogen cleavage by plasma kallikrein, which results in the generation of cleaved high molecular-weight kininogen (HKc) and the release of bradykinin. Higher rate of HMW-kininogen and elevated levels of plasma kallikrein activity has been recorded in the plasma of Alzheimer s disease subjects and changes HMW and cleaved kininogen levels have been significantly correlated with dementia and neuritic plaque scores. Clone 4B12 detects only the cleaved form (LMW) of kininogen (HKc) and not the HMW form (HKi). (Ref.: Yamamoto-Imoto, H., et al (2018). Alzheimer. Dement. (Amst). 10; 480-489; Xu, J., et al. (2018). J. Exp. Clin. Cancer Res. 37; Article 180).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Kininogen-1, cleaved form, clone 4B12, Cat. No. MABN2619, is a mouse monoclonal antibody that detects Kininogen-1 and is tested for use in ELISA, Immunohistochemistry (Paraffin), and Immunoprecipitation.
Key Applications
  • ELISA
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
Application NotesEnzyme Immunoassay (ELISA) Analysis: A representative lot detected Kininogen-1 in ELISA applications (Yamamoto-Imoto, H., et. al. (2018). Alzheimers Dement (Amst). 10:480-489).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Kininogen-1 in Immunoprecipitation applications (Yamamoto-Imoto, H., et. al. (2018). Alzheimers Dement (Amst). 10:480-489).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal region of human Kininogen-1.
Clone4b12
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 4B12 is a mouse monoclonal antibody that detects human Kininogen-1. It targets an epitope within 19 amino acids from the C-terminal region. It recognizes only the cleaved form.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • KNG1
  • BDK
  • KNG
Purification MethodProtein G purified
UniProt Number
Molecular Weight71.96 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human kidney tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Kininogen-1 in human kidney tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABN2619-25UL 04061841440772

Documentation

Anti-Kininogen-1 Antibody, cleaved form Antibody, clone 4B12 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Kininogen-1 Antibody, cleaved form Antibody, clone 4B12 Certificates of Analysis

TitleLot Number
Anti-Kininogen-1, cleaved form, clone 4B12 - Q3416292 Q3416292

References

Reference overviewPub Med ID
A novel detection method of cleaved plasma high-molecular-weight kininogen reveals its correlation with Alzheimer's pathology and cognitive impairment.
Yamamoto-Imoto, H; Zamolodchikov, D; Chen, ZL; Bourne, SL; Rizvi, S; Singh, P; Norris, EH; Weis-Garcia, F; Strickland, S
Alzheimers Dement (Amst)  10  480-489  2018

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