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MABN685 Anti-KDM4A Antibody, clone5H1

Anti-KDM4A Antibody, clone5H1 is a highly specific mouse monoclonal antibody, that targets Jumonji domain-containing protein (JMJD) & has been tested in western blotting, IHC & Immunofluorescence.

Anti-KDM4A Antibody, clone5H1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: JMJD2, JHDM3A, JMJD2A, KIAA0677, KDM4A UniProt Number: O75164
MABN685
100 µL  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHC, IFMAscitesMonoclonal Antibody
Description
Catalogue NumberMABN685
DescriptionAnti-KDM4A Antibody, clone5H1
Alternate Names
  • Lysine-specific demethylase 4A
  • JmjC domain-containing histone demethylation protein 3A
  • Jumonji domain-containing protein 2A
  • KDM4A
Background InformationLysine methylation of histones is associated with both transcriptionally active chromatin and with silent chromatin, depending on what residue is modified. Histone methyltransferases and demethylases ensure that histone methylations are dynamic and can vary depending on cell cycle or developmental stage. KDM4A demethylates H3K36me3, a modification enriched in the 3′ end of active genes. KDM4A is also known as Lysine-specific demethylase 4A or JmjC domain-containing histone demethylation protein 3A, or Jumonji domain-containing protein 2A, and is encoded by the human gene named KDM4A/JHDM3A/JMJD2/JMJD2A/KIAA0677. KDM4A is the histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone gene repression, and KDM4A functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated forms. KDM4A does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20', but KDM4A does demethylate trimethylated H3 'Lys-9' and H3 'Lys-36' residues, while it has no activity on mono- and dimethylated residues. KDM4A also plays a critical role in muscle differentiation as demethylation of various targets allows influences the expression of the Myog gene family critical in muscle cell development. KDMA4A also interacts with histone deacetylases HDAC1, HDAC2, and HCAC3. It is localized in the nucleus and widely expressed. KDM4A’s own levels are mediated via ubiquitination and proteasome degradation. EMD-Millipore’s Anti-KDM4A mouse monoclonal antibody has been tested in western blot on HEK293 and KDM4A transfected HEK293 cell lysates and in paraffin embedded immunohistochemistry on human colon and larynx cancer tissues and in fluorescent immunocytochemistry on NTERA-2 cells in culture.
References
Product Information
FormatAscites
Control
  • HEK293 and KDM4A-hIgGFc transfected HEK293 cell lysates
PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-KDM4A Antibody, clone5H1 is a highly specific mouse monoclonal antibody, that targets Jumonji domain-containing protein (JMJD) & has been tested in western blotting, IHC & Immunofluorescence.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunofluorescence
Application NotesImmunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected KDM4A in human colon cancer and larynx cancer tissues.

Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected KDM4A in NTERA-2 cells.

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenPurified recombinant fragment of human KDM4A expressed in E. Coli.
Clone5H1
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • JMJD2
  • JHDM3A
  • JMJD2A
  • KIAA0677
  • KDM4A
Purification MethodUnpurified
UniProt Number
Molecular Weight~72 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 and KDM4A-hIgGFc transfected HEK293 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected KDM4A-hIgGFc transfected HEK293 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABN685 04053252917509