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09-864 Anti-JHDM1B Antibody

09-864
100 µg  
Purchase on Sigma-Aldrich

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Áttekintés

Replacement Information

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Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number09-864
DescriptionAnti-JHDM1B Antibody
Alternate Names
  • JMJD
  • lysine (K)-specific demethylase 2B
  • F-box protein FBL10
  • [Histone-H3]-lysine-36 demethylase 1B
  • JEMMA (Jumonji domain, EMSY-interactor, methyltransferase motif) protein
  • F-box and leucine-rich repeat protein 10
  • protein containing CXXC domain 2
  • jumonji C domain-containing histone demethylase 1B
  • Jumonji domain-containing EMSY-interactor methyltransferase motif protein
  • lysine-specific demethylase 2B
  • Protein-containing CXXC domain 2
  • JmjC domain-containing histone demethylation protein 1B
  • Protein JEMMA
  • F-box/LRR-repeat protein 10
  • CXXC-type zinc finger protein 2
Background InformationJmjC domain-containing histone demethylation protein 1B, also known as Lysine-specific demethylase 2B, is a nuclear enzyme encoded by the JHDM1B gene (also known as KDM2B) in human. JHDM1A/KDM2B exhibits H3K36-specific demethylase activity with a preference for H3K36me2 over H3K36me and H3K36me3 (PMID 16362057). JHDM1A/KDM2B is also reported to negatively regulate cell proliferation by targeting rDNA and thereby repressing the transcription of rRNA in a JmjC-domain-dependent manner (PMID 17994099).
References
Product Information
FormatAffinity Purified
Control
  • HeLa nuclear extract
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-JHDM1B Antibody is a rabbit polyclonal antibody for detection of JHDM1B also known as lysine (K)-specific demethylase 2B, F-box protein FBL10, [Histone-H3]-lysine-36 demethylase 1B & has been validated in WB, ICC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected JHDM1B in NIH/3T3 and HeLa cells.
Immunocytochemistry Analysis: 2 µg/mL from a representative lot detected JHDM1B in A431 cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human JHDM1B.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Rabbit, Chimpanzee, Horse, and Porcine based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq].
Gene Symbol
  • KDM2B
  • CXXC2
  • FBXL10
  • JHDM1B
  • PCCX2
  • Fbl10
  • FBL10
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~165 kDa observed. An uncharacterized lower molecular weight band may be observed in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa nuclear extract.

Western Blot Analysis: 1 µg/mL of this antibody detected JHDM1B on 10 µg of HeLa nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
09-864 04053252415302

Documentation

Anti-JHDM1B Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-JHDM1B Antibody Certificates of Analysis

TitleLot Number
Anti-JHDM1B 2465308
Anti-JHDM1B - 2115674 2115674
Anti-JHDM1B - 2154114 2154114
Anti-JHDM1B - 2360002 2360002
Anti-JHDM1B - 2395802 2395802
Anti-JHDM1B - 1978006 1978006
Anti-JHDM1B - 2196078 2196078
Anti-JHDM1B - 2278557 2278557
Anti-JHDM1B - 2300018 2300018
Anti-JHDM1B - 3173542 3173542

References

Reference overviewApplicationPub Med ID
The KDM2B- let-7b -EZH2 axis in myelodysplastic syndromes as a target for combined epigenetic therapy.
Karoopongse, E; Yeung, C; Byon, J; Ramakrishnan, A; Holman, ZJ; Jiang, PY; Yu, Q; Deeg, HJ; Marcondes, AM
PloS one  9  e107817  2014

Kivonat megmutatása
Western Blotting25225797 25225797
Fbxl10/Kdm2b deficiency accelerates neural progenitor cell death and leads to exencephaly.
Fukuda, T; Tokunaga, A; Sakamoto, R; Yoshida, N
Mol Cell Neurosci  46  614-24  2010

Kivonat megmutatása
21220025 21220025
IPO8 and FBXL10: new reference genes for gene expression studies in human adipose tissue.
Hurtado del Pozo, C; Calvo, RM; Vesperinas-García, G; Gómez-Ambrosi, J; Frühbeck, G; Corripio-Sánchez, R; Rubio, MA; Obregon, MJ
Obesity (Silver Spring, Md.)  18  897-903  2009

Kivonat megmutatása
19876011 19876011
JHDM1B/FBXL10 is a nucleolar protein that represses transcription of ribosomal RNA genes.
Frescas, D; Guardavaccaro, D; Bassermann, F; Koyama-Nasu, R; Pagano, M
Nature  450  309-13  2007

Kivonat megmutatása
17994099 17994099
Polycomb group and SCF ubiquitin ligases are found in a novel BCOR complex that is recruited to BCL6 targets.
Gearhart, MD; Corcoran, CM; Wamstad, JA; Bardwell, VJ
Molecular and cellular biology  26  6880-9  2005

Kivonat megmutatása Teljes cikk
16943429 16943429

Brochure

Title
NPI Flyer- Epigenetics and Nuclear Function Feature