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ABE1932 Anti-IRX-5

This rabbit polyclonal Anti-IRX-5 Antibody, Cat. No. ABE1932 detects levels of IRX-5, and has been published and validated for use in Western Blotting.

Anti-IRX-5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: Irx5, Irxb2 UniProt Number: Q9JKQ4
ABE1932
100 µL  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
MWBRbUnpurifiedPolyclonal Antibody
Description
Catalogue NumberABE1932
DescriptionAnti-IRX-5
Alternate Names
  • Iroquois-class homeodomain protein IRX-5
  • Homeodomain protein IRXB2
  • Iroquois homeobox protein 5
Background InformationIroquois-class homeodomain protein IRX-5 (UniProt Q9JKQ4; also known as Homeodomain protein IRXB2, Iroquois homeobox protein 5) is encoded by the Irx5 (also known as Irxb2) gene (Gene ID 54352) in murine species. Iroquois homeobox (Irx) genes encode a family of homeodomain transcription factors that are conserved from worms to vertebrates. Six IRX genes are located in two clusters in human (IRXA and IRXB) and mouse (IrxA and IrxB) genomes. Irx3 and Irx5 of the IrxB cluster are expressed in the developing mouse limb bud and are essential for proximal and anterior skeletal formation. Irx3 and Irx5 are required to establish early anteroposterior (AP) polarity and specify the anterior progenitor population. Individual Irx genes are also important for certain postnatal cardiac functions in the developing mouse heart, including cardiac repolarization (Irx5) and rapid ventricular conduction (Irx3). Postnatal deletion of Irx3 and Irx5 in the myocardium leads to prolongation of atrioventricular conduction, due in part to activation of expression of the Na(+) channel protein Nav1.5. However, combined postnatal loss of Irx3 and Irx5 results in a restoration of the repolarization gradient that is altered in Irx5 mutant hearts, suggesting that postnatal Irx3 activity can be repressed by Irx5 in mice.
References
Product Information
FormatUnpurified
PresentationRabbit polyclonal antibody serum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis rabbit polyclonal Anti-IRX-5 Antibody, Cat. No. ABE1932 detects levels of IRX-5, and has been published and validated for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot was affinity purified and detected an increased IRX-5/IRXB2 expression in postnatal P10 heart tissue lysates from miR-1-2-knockout mice (Zhao, Y., et al. (2007). Cell. 129(2):303-317).
Biological Information
ImmunogenHis-tagged recombinant murine IRX-5/IRXB2 C-terminal fragment GST fusion protein (Chen, C.W., et al. (2005). Dev. Biol. 287(1):48-60).
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antiserum targets an internal region within the C-terminal half of murine IRX-5/IRXB2.
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Irx5
  • Irxb2
Purification MethodUnpurified.
UniProt Number
Molecular Weight~54 kDa observed. 50.76 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in postnatal mouse heart tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antiserum detected IRX-5/IRXB2 in 10 µg of postnatal P10 mouse heart tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
ABE1932 04054839034633