Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

07-1213 Anti-IP3 Receptor 1 Antibody

This Anti-IP3 Receptor 1 Antibody is validated for use in IH(P), WB for the detection of IP3 Receptor 1.

Anti-IP3 Receptor 1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Lipid Signaling Gene Symbol: INSP3R1, IP3R, IP3R1, InsP3R1, Insp3r1, SCA15, SCA16 UniProt Number: Q14643
07-1213
100 µL  
Purchase on Sigma-Aldrich

Különleges ajánlatok

Áttekintés

Replacement Information

Különleges ajánlatok

100% Performance Guaranteed

Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, H, Ca, RIH(P), WBRbSerumPolyclonal Antibody
Description
Catalogue Number07-1213
ReplacesAB9072
DescriptionAnti-IP3 Receptor 1 Antibody
Alternate Names
  • IP3 receptor isoform 1
  • Type 1 InsP3 receptor
  • Type 1 inositol 1,4,5-trisphosphate receptor
  • inositol 1,4,5-triphosphate receptor, type 1
  • spinocerebellar ataxia 15
  • spinocerebellar ataxia 16
Background InformationIP3 Receptor 1 is an intracellular channel that when stimulated by inositol 1,4,5-triphosphate mediates the release of calcium from the endoplasmic reticulum. Defects within the gene ITPR1 cause a group of cerebellar disorders known as spinocerebellar ataxia type 15 (SCA15). This receptor is widely expressed in tissue.
References
Product Information
FormatSerum
HS Code3002 15 90
Control
  • C2C12 cell lysates
PresentationRabbit serum. Liquid
Quality LevelMQ100
Applications
ApplicationThis Anti-IP3 Receptor 1 Antibody is validated for use in IH(P), WB for the detection of IP3 Receptor 1.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A 1:100 dilution of this antibody detected IP3 Receptor 1 in mouse cerebellum tissue.
Biological Information
ImmunogenSynthetic peptide from the N-terminal of human IP3 Receptor 1.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThe antibody reacts with the 300 kDa IP3 Receptor 1 protein.
Isotypemixed IgGs
Species Reactivity
  • Mouse
  • Human
  • Canine
  • Rat
Species Reactivity NoteProven to react with mouse and human. Expected to react with canine and rat based on sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • INSP3R1
  • IP3R
  • IP3R1
  • InsP3R1
  • Insp3r1
  • SCA15
  • SCA16
Purification MethodUnpurified rabbit serum
UniProt Number
UniProt SummaryFUNCTION: Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate.
SUBUNIT STRUCTURE: Homotetramer. Interacts with TRPC4. The PPXXF motif binds HOM1, HOM2 and HOM3. Interacts with RYR1, RYR2, ITPR1, SHANK1 and SHANK3. Interacts with ERP44 in a pH-, redox state- and calcium-dependent manner which results in the inhibition the calcium channel activity. The strength of this interaction inversely correlates with calcium concentration. Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1. Interacts with AHCYL1 By similarity. Interacts with MRVI1 and CABP1 (via N-terminus).
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Widely expressed.
DOMAIN: The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region.
PTM: Phosphorylated by cAMP kinase. Phosphorylation prevents the ligand-induced opening of the calcium channels By similarity.
Phosphorylated on tyrosine residues.
INVOLVEMENT IN DISEASE: Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15) (SCA15) [MIM:606658]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA15 is an autosomal dominant cerebellar ataxia (ADCA). It is very slow progressing form with a wide range of onset, ranging from childhood to adult. Most patients remain ambulatory.
MISCELLANEOUS: Calcium appears to inhibit ligand binding to the receptor, most probably by interacting with a distinct calcium-binding protein which then inhibits the receptor.
SEQUENCE SIMILARITIES: Belongs to the InsP3 receptor family.
Contains 5 MIR domains.
Molecular WeightApprox. 300 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in C2C12 cell lysates.
Western Blot Analysis: 1:500 dilution of this lot detected IP3 RECPT 1 on 10 ug of C2C12 lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 1 year date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
07-1213 04053252281846

Documentation

Anti-IP3 Receptor 1 Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-IP3 Receptor 1 Antibody Certificates of Analysis

TitleLot Number
Anti- IP3 Receptor 1 - 2119010 2119010
Anti- IP3 Receptor 1 - 2128259 2128259
Anti- IP3 Receptor 1 - 1945620 1945620
Anti- IP3 Receptor 1 - 2198975 2198975
Anti- IP3 Receptor 1 - 2324677 2324677
Anti- IP3 Receptor 1 - 3232423 3232423
Anti- IP3 Receptor 1 - 3319800 3319800
Anti- IP3 Receptor 1 - 3546915 3546915
Anti- IP3 Receptor 1 - 3709632 3709632
Anti- IP3 Receptor 1 - 3733813 3733813
Show more