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MABF1478-25UG Anti-IFNGR1/CD119 Antibody, clone 2E2

Anti-IFNGR1/CD119, clone 2E2, Cat. No. MABF1478, is an Armenian hamster monoclonal antibody that detects IFNGR1/CD119 and is tested for use in Flow Cytometry.

ÚjMABF1478-25UG
25 μg  
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Replacement Information
Description
Catalogue NumberMABF1478-25UG
DescriptionAnti-IFNGR1/CD119 Antibody, clone 2E2
Alternate Names
  • Interferon gamma receptor 1
  • IFN-gamma receptor 1
  • IFN-gamma-R1
  • Interferon gamma receptor alpha-chain
  • IFN-gamma-R-alpha
  • CD119
Background InformationInterferon gamma receptor 1 (UniProt: P15261; also known as IFN-gamma receptor 1, IFN-gamma-R1, Interferon gamma receptor alpha-chain, IFN-gamma-R-alpha, CD119) is encoded by the Ifngr1 (also known as Ifngr) gene (Gene ID: 15979) in murine species. IFNGR1/CD119 is single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 26-254), a transmembrane domain (aa 255-275), and a cytoplasmic domain (aa 276-477). It serves as a receptor subunit for interferon- that plays critical roles in antibacterial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. It associates with IFNGR2 to form a functional receptor. Upon ligand binding, the intracellular domain of IFNGR1/CD119 opens out to allow association of downstream signaling components JAK1 and JAK2. In turn, the activated JAK1 phosphorylates IFNGR1/CD119 to form a docking site for STAT1. Subsequent phosphorylation of STAT1 leads to its dimerization, translocation to the nucleus, and stimulation of target gene transcription. Phosphorylation of the intracellular domain of IFNGR1/CD119 also provides a docking site for SOCS1 that regulates the JAK-STAT pathway by competing with STAT1 binding to IFNGR1. (Ref.: Wei, B., et al. (2010). Am. J. Transplant. 10(1); 69-80; Haring, JS., et al. (2005). J. Immunol. 174(11); 6791-6802; Qing, Y., et al. (2005). J. Biol. Chem. 280(3); 1849-1853).
References
Product Information
FormatPurified
PresentationPurified armenian hamster monoclonal antibody IgG2-IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-IFNGR1/CD119, clone 2E2, Cat. No. MABF1478, is an Armenian hamster monoclonal antibody that detects IFNGR1/CD119 and is tested for use in Flow Cytometry.
Key Applications
  • Flow Cytometry
Application NotesTested Applications

Flow Cytometry Analysis: A representative lot detected IFNGR1/CD119 in Flow Cytometry applications (Haring, J.S., et al. (2005). J Immunol. 174(11):6791-802).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenRecombinant soluble form of -chain of mouse IFNGR1/CD119.
EpitopeExtracellular domain
Clone2E2
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostArmenian Hamster
SpecificityClone 2E2 is an Armenian hamster monoclonal antibody that detects Interferon 1 receptor (IFNGR1/CD119). It targets an epitope within the extracellular domain.
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Ifngr1
Purification MethodProtein G purified
UniProt Number
Molecular Weight52.34 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in C57BL/6 Mouse splenocytes.

Flow Cytometry Analysis: 1.0 µg of this antibody detected IFNGR1/CD119 in one million C57BL/6 Mouse splenocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABF1478-25UG 04065270937919