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382160 Anti-Histone H4, Acetylated (2-19) Rabbit pAb

Anti-Histone H4, Acetylated (2-19) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
382160
  
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Áttekintés

Kulcsspecifikációk táblázata

Species ReactivityHostAntibody Type
H, TRbPolyclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~10 kDa acetylated histone H4 protein in Tetrahymena and sodium butyrate-treated HeLa cells.
Catalogue Number382160
Brand Family Calbiochem®
Application Data
Detection of human acetylated histone H4 by immunoblotting. Samples: Acid-extracted proteins from HeLa cells treated with sodium butyrate at antibody concentrations of 0.5 µg/ml (lane 1) and 2 µg/ml (lane 2). Primary antibody: Anti-Histone H4, Acetylated (2-19) Rabbit pAb (Cat. No. 382160) (2 µg/ml). Detection: chemiluminescence.
References
ReferencesBraunstein, M., et al. 1996. Mol. Cell. Biol. 16, 4349.
Braunstein, M., et al. 1993. Genes Devel. 7, 592.
Perry, C.A., et al. 1993. Biochemistry 32, 13605.
Lin, R., et al. 1989. J. Cell. Biol. 108, 1577.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.
Positive controlTetrahymena and sodium butyrate-treated HeLa cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
Application NotesImmunoblotting (0.5-2 µg/ml)
Immunocytochemistry (5-10 µg/ml)
Immunoprecipitation (5µg/500 µg lysate)
Application CommentsThe antibody detected acetylated histone H4 in acid-extracted proteins from HeLa cells. Cross-reacts with acetylated histone H2B from Tetrahymena and weakly cross-reacts with histone H2B from HeLa cells. May cross-react with other acetylated proteins. Using sodium butyrate, an inhibitor of histone deacetylases, enhances detection of acetylated histone H4. For immunocytochemistry, human A431 cells were treated with 5 mM sodium butyrate, an inhibitor of histone deacetylases, for 24 h and fixed with ice-cold 1% paraformaldehyde in PBS. Variables associated with assay conditions will dictate the optimal dilution.
Biological Information
Immunogena tetra-acetylated synthetic peptide [AGGAcKGGAcKGMGAcKVGAAcKRHS(C)] corresponding to amino acids 2-19 of Tetrahymena histone H4, conjugated to KLH
ImmunogenTetrahymena
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Tetrahymena
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Global Trade Item Number
Katalógusszám GTIN
382160 0