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MABC675 Anti-HSPA5 Antibody, clone 4E3

Anti-HSPA5 Antibody, clone 4E3 is a highly specific mouse monoclonal antibody, that targets GRP-78 & has been tested in western blotting & IHC.

Anti-HSPA5 Antibody, clone 4E3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: HSPA5, GRP78 UniProt Number: P11021
MABC675
100 µL  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, MWB, IHCMAscitesMonoclonal Antibody
Description
Catalogue NumberMABC675
DescriptionAnti-HSPA5 Antibody, clone 4E3
Alternate Names
  • 78 kDa glucose-regulated protein
  • GRP-78
  • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
  • Heat shock 70 kDa protein 5
  • Immunoglobulin heavy chain-binding protein
  • BiP
Background InformationThe gene HSPA5 encodes a protein named GRP-78 (78 kDa glucose-regulated protein) also known as BiP (immunoglobulin heavy chain-binding protein) or heat shock 70 kDa protein 5. While originally discovered as one of set of genes that were induced upon cellular starvation and stress, HSPA5 encoded GRP-78 is now known to be an important member of the heat-shock family of proteins. The HSPA5 encoded GRP-78 protein appears to play a role in helping in the assembly of multimeric protein complexes in the endoplasmic reticulum (ER). Cellular localization is in the endoplasmic reticulum lumen and appears as cytoplasmic-like staining. The HSPA5 encoded protein is an essential component of the translocation processes within the endoplasmic reticulum and also functions in the retrograde transport across the ER membrane of incorrectly folded proteins destined to be degraded by the ubiquitin proteasome system. The HSPA5 encoded protein, while widely expressed in all cells types under normal conditions, is particularly induced under conditions of cellular stress, heat, or toxins and conditions which lead to the accumulation of unfolded proteins in the ER. EMD-Millipore’s Anti-HSPA5 protein mouse monoclonal antibody, clone 4E3, has been tested by western blot in human HeLa and Jurkat cell lysates as well as mouse NIH3T3 cell lysates. The clone has also been successfully tested in immunohistochemistry in human, paraffin-embedded, thyroid tissues.
References
Product Information
FormatAscites
Control
  • HeLa cell lysate
PresentationMouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-HSPA5 Antibody, clone 4E3 is a highly specific mouse monoclonal antibody, that targets GRP-78 & has been tested in western blotting & IHC.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern Blotting Analysis: A 1:500-2,000 dilution of this antibody detected HSPA5 in 10 µg of NIH/3T3, Jurkat, and A431 cell lysates.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected HSPA5 in rat cerebellum, rat pons/midbrain, rat hippocampus, and human thyroid tissues.

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenPurified recominant fragment of human HSPA5 expressed in E. coli.
Clone4E3
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
  • Rat
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • HSPA5
  • GRP78
Purification MethodUnpurified
UniProt Number
Molecular Weight~72 kDa observed. Uncharacterized bands may appear in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected HSPA5 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABC675 04053252910012

Documentation

Anti-HSPA5 Antibody, clone 4E3 MSDS

Title

Safety Data Sheet (SDS) 

Anti-HSPA5 Antibody, clone 4E3 Certificates of Analysis

TitleLot Number
Anti-HSPA5, clone 4E3 - VP1401281 VP1401281
Anti-HSPA5, clone 4E3 - VP1911082 VP1911082
Anti-HSPA5, clone 4E3 - VP2106031 VP2106031
Anti-HSPA5, clone 4E3 -VP1410288 VP1410288
Anti-HSPA5, clone 4E3 -VP1505224 VP1505224
Anti-HSPA5, clone 4E3 -VP1511120 VP1511120
Anti-HSPA5, clone 4E3 -VP1601278 VP1601278
Anti-HSPA5, clone 4E3 -VP1602240 VP1602240
Anti-HSPA5, clone 4E3 -VP1603021 VP1603021
Anti-HSPA5, clone 4E3 -VP1609039 VP1609039
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