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MABS2068-25UG Anti-HSD3B1 Antibody, clone 4C1

Anti-HSD3B1, clone 4C1, Cat. No. MABS2068, is a mouse monoclonal antibody that detects 3-beta-HSD I (HSD3B1) and has been tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.

Anti-HSD3B1 Antibody, clone 4C1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: HSD3B1, 3BH, HSD3BA UniProt Number: P14060
MABS2068-25UG
25 μg  
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Replacement Information

Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
HELISA, IH(P), WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS2068-25UG
DescriptionAnti-HSD3B1 Antibody, clone 4C1
Alternate Names
  • 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
  • 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I
  • 3-beta-HSD I
  • Trophoblast antigen FDO161G
Background Information3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 (UniProt: P14060; also known as 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I, 3-beta-HSD I, Trophoblast antigen FDO161G) is encoded by the HSD3B1 (also known as 3BH, HSDB3A) gene (Gene ID: 3283) in human. HSD3B1 is a single -pass membrane protein localized in mitochondria and endoplasmic reticulum. Its transmembrane region is localized to amino acids 288-308. It is a bifunctional enzyme that catalyzes the oxidative conversion of Delta 5-ene-3-beta-hydroxy steroid and causes oxidative conversion of ketosteroids. It plays a vital role in the biosynthesis of all classes of hormonal steroids. Humans have two HSD3B isozymes. HSD3B1 is mainly expressed in the placenta, mammary and, prostate glands, and other peripheral tissues including the skin and adipose tissue and HSD3B2 is expressed primarily in the adrenal and gonads. They exhibit about 93% sequence homology. (Ref.: Gomez-Sanchez, CE et al. (2017). Steroids 127; 56-61).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-HSD3B1, clone 4C1, Cat. No. MABS2068, is a mouse monoclonal antibody that detects 3-beta-HSD I (HSD3B1) and has been tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • ELISA
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected HSD3B1 in human adrenal gland tissue sections.

ELISA Analysis : A representative lot detected HSD3B1 in ELISA applications (Gomez-Sanchez, C.E., et. al. (2017). Steroids. 127:56-61).

Western Blotting Analysis: 1 µg/mL from a representative lot detected HSD3B1 in human placenta tissue lysate in Western blotting applications.

Immunohistochemistry (Paraffin) Analysis: A representative lot detected HSD3B1 in Immunohistochemistry applications (Gomez-Sanchez, C.E., et. al. (2017). Steroids. 127:56-61).

Western Blotting Analysis: A representative lot detected HSD3B1 in Western Blotting applications (Gomez-Sanchez, C.E., et. al. (2017). Steroids. 127:56-61).
Biological Information
ImmunogenA KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of human 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 (HSD3B1).
EpitopeN-terminus
Clone4C1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 4C1 detects 3-beta-HSD I (HSD3B1) in human tissues. It targets an epitope with in 14 amino acids from the N-terminal region.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • HSD3B1
  • 3BH
  • HSD3BA
Purification MethodProtein G purified
UniProt Number
Molecular Weight~38 kDa observed; 42.25 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human placenta tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected HSD3B1 in human placenta tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABS2068-25UG 04054839551062