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MABF2162-100UG Anti-HLA-F Antibody, clone 3D11

Anti-HLA-F, clone 3D11, Cat. No. MABF2162, is a mouse monoclonal antibody that detects HLA class I histocompatibility antigen, alpha chain F and has been tested for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.

MABF2162-100UG
100 μg  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue NumberMABF2162-100UG
DescriptionAnti-HLA-F Antibody, clone 3D11
Alternate Names
  • HLA class I histocompatibility antigen alpha chain F
  • CDA12
  • HLA F antigen
  • Leukocyte antigen F
  • MHC class I antigen F
Background InformationHLA class I histocompatibility antigen, alpha chain F (UniProt: P30511; also known as CDA12, HLA F antigen, Leukocyte antigen F, MHC class I antigen F) is encoded by the HLA-F (also known as HLA-5.4, HLAF) gene (Gene ID: 3134) in human. HLA-F is a single-pass type I membrane heterodimeric protein that is involved in the presentation of foreign antigens to the immune system. It is expressed in B cells, NK cells, monocytes, and T cells and its expression is reported to be coincident with the activated immune response. It is mainly localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off. The mature form has an extracellular domain (aa 22-305), a transmembrane domain (aa 306-329), and a short cytoplasmic domain (aa 330-346). The HLA-F locus has very low allelic polymorphism and is highly conserved in distantly related non-human primates indicating its conserved function. It is involved in the regulation of immune system in pregnancy, infection, and autoimmunity by signaling through NK cell receptors. (Ref.: Dulberger, CL et al. (2017). Immunity 46(6); 1018 1029; Lee, N., et al. (2010). Eur. J. Immunol. 40(8): 2308 2318).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-HLA-F, clone 3D11, Cat. No. MABF2162, is a mouse monoclonal antibody that detects HLA class I histocompatibility antigen, alpha chain F and has been tested for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • ELISA
  • Flow Cytometry
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesELISA Analysis: A representative lot detected HLA-F in ELISA applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18).

Western Blotting Analysis: A representative lot detected HLA-F in Western Blotting applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18; Ishitani, A., et. al. (2003). J Immunol. 171(3):1376-84).

Flow Cytometry Analysis: A representative lot detected HLA-F in Flow Cytometry applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18).

Immunohistochemistry Analysis: A representative lot detected HLA-F in Immunohistochemistry applications (Ishitani, A., et. al. (2003). J Immunol. 171(3):1376-84).
Biological Information
ImmunogenFull-length human recombinant HLA-F Heavy chains (excluding the signal sequence).
Clone3D11
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 3D11 specifically detects human HLA-F. It targets an epitope with in the alpha 2 domain from the N-terminal half.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • HLA-F
  • HLA-5.4
  • HLAF
Purification MethodProtein G purified
UniProt Number
Molecular Weight39.06 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human placenta tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected HLA-F in human placenta tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABF2162-100UG 04054839577178

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