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MABS1190 Anti-Glucokinase/GCK Antibody, clone 9G1.2

Anti-GlucokinaseAntibody, clone 9G1.2 is an antibody against GCK for use in Western Blotting, Immunohistochemistry (Paraffin).

Anti-Glucokinase/GCK Antibody, clone 9G1.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Glucose/Glycogen Metabolism Gene Symbol: GCK, HHF3, MODY2 UniProt Number: P35557
MABS1190
100 μg  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1190
DescriptionAnti-Glucokinase/GCK Antibody, clone 9G1.2
Alternate Names
  • Glucokinase
  • GK
  • Hexokinase-4
  • Hexokinase-D
  • Hexokinase type IV
  • HK IV
  • HK4
Background InformationGlucokinase (EC 2.7.1.2; UniProt P35557; also known as GK, Hexokinase-4, Hexokinase-D, Hexokinase type IV, HK IV, HK4) is encoded by the GCK (also known as HHF3, MODY2) gene (Gene ID 2645) in human. Glucokinase/GK functions as the body's primary glucose sensor by catalyzing the ATP-dependent phosphorylation of glucose to glucose-6-phosphate, the rate-limiting step of glucose metabolism in human pancreas and liver. GK activity is regulated rapidly in response to the elevated blood glucose after feeding, whereby a change in GK conformation causes a dissociation of the nuclear GK-glucokinase regulatory protein (GKRP) complex, allowing GK translocation to the cytoplasm in an activated state. In mammals, there exist four types of hexokinases, HK1, HK2, and HK3 have high affinity for glucose, while GK (also known as HK4) has low affinity for glucose and its expression is restricted to the pancreas and liver. GCK gene mutations cause Maturity-onset diabetes of the young 2 (MODY2) and Familial hyperinsulinemic hypoglycemia 3 (HHF3).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-GlucokinaseAntibody, clone 9G1.2 is an antibody against GCK for use in Western Blotting, Immunohistochemistry (Paraffin).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Glucokinase/GCK in human liver, colon, and tonsil tissue sections.
Biological Information
ImmunogenGST-tagged recombinant internal fragment from the C-terminal half of human Glucokinase/GCK.
EpitopeInternal (C-terminal half).
Clone9G1.2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 9G1.2 recognizes an epitope present in all three spliced isoforms of human Glucokinase/GCK reported by UniProt (P35557).
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • GCK
  • HHF3
  • MODY2
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~52 kDa observed. ~52 kDa observed. 52.19 kDa (isoform 1), 52.13 kDa (isoform 2), and 52.04 kDa (isoform 3) calculated. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Glucokinase/GCK in 10 µg of HepG2 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABS1190 04055977343861