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MABC667 Anti-GPC3 Antibody, clone 9C2

Detect Glypican using this mouse monoclonal antibody, Anti-GPC3 Antibody, clone 9C2 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry.

Anti-GPC3 Antibody, clone 9C2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: GPC3, OCI5 UniProt Number: P51654
MABC667
100 µL  
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Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IF, IHC, FCMAscitesMonoclonal Antibody
Description
Catalogue NumberMABC667
DescriptionAnti-GPC3 Antibody, clone 9C2
Alternate Names
  • SGB
  • DGSX
  • MXR7
  • SDYS
  • SGBS
  • OCI-5
  • SGBS1
  • GTR2-2
  • Glypican-3
  • Intestinal protein OCI-5
Background InformationGlypican-3 or GTR2-2 is the protein product of the gene GPC3. It is a cell surface heparin sulfate containing glycoprotein that plays an important role in the modulation of growth and development of mesodermal tissues and organs, as well as various cancers. Among its many known functions, it is a regulator of the dipeptidyl peptidase DPP4 (CD26). DPP4 plays an important role in glucose metabolism via its action on incretin and GLP-1 hormones. DPP4 also is a cancer marker and thought to function as a cancer suppressor. Thus acting as a regulator of DPP4, Glypican-3 (GPC3) participates in everything from glucose and blood sugar regulation to tumor growth. Glypican-3 (GPC3) is most highly expressed in the lung, liver and kidney, and typically a secreted form, secreted glypican-3, is found in the extracellular space generated as a cleavage product by the action of a convertase. Defects in GPC3 cause Simpson-Golabi-Behmel syndrome type 1 (SGBS1) or Simpson dysmorphia syndrome (SDYS). This is a condition characterized by pre-and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. EMD-Millipore’s anti-GPC3 mouse monoclonal antibody, clone 9C2, has been tested in western blot against recombinant human protein, human HepG2, HEK293, Jurkat, and SK-N-SH cell line lysates, as well as rat PC-12 and mouse liver cell lysates. In addition, the clone as been successfully used in paraffin-embedded immunohistochemistry on human liver and breast cancer tissues and in immunofluorescent immunocytochemistry on human HeLa cells in culture. Finally, the clone has been tested successfully on human cells by flow cytometry and against the recombinant antigen by ELISA.
References
Product Information
FormatAscites
Control
  • Mouse liver, HepG2, HEK293, Jurkat, SK-N-SH, PC-12, and F9 cell lysates
PresentationPurified mouse monoclonal in PBS with 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationDetect Glypican using this mouse monoclonal antibody, Anti-GPC3 Antibody, clone 9C2 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry.
Key Applications
  • Western Blotting
  • Immunofluorescence
  • Immunohistochemistry
  • Flow Cytometry
Application NotesImmunohistochemistry Analysis: A 1:100 dilution from a representative lot detected GPC3 in human liver and kidney tissues.

Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected GPC3 in HeLa cells.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected GPC3 in Jurkat cells.

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenPurified recombinant fragment of human GPC3 expressed in E. Coli.
Clone9C2
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Gene Symbol
  • GPC3
  • OCI5
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~66 kDa observed. Uncharacterized bands may appear in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse liver, HepG2, HEK293, Jurkat, SK-N-SH, PC-12, and F9 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected GPC3 in mouse liver, HepG2, HEK293, Jurkat, SK-N-SH, PC-12, and F9 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABC667 04053252918056

Documentation

Anti-GPC3 Antibody, clone 9C2 MSDS

Title

Safety Data Sheet (SDS) 

Anti-GPC3 Antibody, clone 9C2 Certificates of Analysis

TitleLot Number
Anti-GPC3, clone 9C2 - VP1401035 VP1401035
Anti-GPC3, clone 9C2 - VP1404281 VP1404281
Anti-GPC3, clone 9C2 - QVP1305307 QVP1305307
Anti-GPC3, clone 9C2 - VP1905161 VP1905161
Anti-GPC3, clone 9C2 - VP1909200 VP1909200
Anti-GPC3, clone 9C2 - VP2112011 VP2112011
Anti-GPC3, clone 9C2 - VP2202171 VP2202171
Anti-GPC3, clone 9C2 - VP2205192 VP2205192
Anti-GPC3, clone 9C2 - VP2210043 VP2210043
Anti-GPC3, clone 9C2 - VP2303020 VP2303020
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