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MABE2033-25UG Anti-GINS1 Antibody, clone 192B

Anti-GINS1, clone 192B, Cat. No. MABE2033, is a mouse monoclonal antibody that detects GINS complex subunit 1 and is tested for use in Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

MABE2033-25UG
25 μg  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue NumberMABE2033-25UG
DescriptionAnti-GINS1 Antibody, clone 192B
Alternate Names
  • DNA replication complex GINS protein PSF1
  • GINS complex subunit 1
Background InformationDNA replication complex GINS protein PSF1 (UniProt: Q14691; also known as GINS complex subunit 1) is encoded by the GINS1 (also known as KIAA0186, PSF1) gene (Gene ID: 9837) in human. GINS1 is a component of the GINS (Go-Ichi-Ni-San) complex, a heterotetramer of GINS1, 2, 3, and 4, that plays an essential role in the initiation of DNA replication and progression of DNA replication forks. GINS complex is highly conserved in mammalian species. Its each subunit is composed of two types of structural domains: an -helix rich (A) domain and a β-strand rich (B) domain. GINS1 is highly expressed in undifferentiated cells and its deficiency in mice is shown to cause embryonic lethality by day 6.5. Its levels are significantly increased in several types of cancers, including lung, breast, and prostate cancers and several cancer cell lines. Mutations in GINS1 gene are known to cause immunodeficiency 5 (IMD55) that is characterized by chronic neutropenia, natural killer cell deficiency, and recurrent viral and bacterial infections, and most patients exhibit growth retardation. (Ref.: Hsieh, H-Y., et al. (2019). Cancer Sci. 111(7); 2400-2412; Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7); 2087-2095).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-GINS1, clone 192B, Cat. No. MABE2033, is a mouse monoclonal antibody that detects GINS complex subunit 1 and is tested for use in Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
Application NotesTested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GINS1 in MOLT-4 and Daudi cell lysates.

Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated GINS1 in Immunoprecipitation applications (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7): 2087-95).

Immunohistochemistry Applications: A representative lot detected GINS1 in Immunohistochemistry applications (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7): 2087-95).

Western Blotting Analysis: A representative lot detected GINS1 in Western Blotting applications (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7): 2087-95).

Immunofluorescence Analysis: A representative lot detected GINS1 in Immunofluorescence applications (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7): 2087-95).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenFull-length recombinant human DNA replication complex GINS protein PSF1 (GINS1).
EpitopeUnknown
Clone192B
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 192B is a mouse monoclonal antibody that detects Human DNA replication complex GINS protein PSF1 (GINS1).
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • GINS1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~23 kDa observed; 22.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GINS1 in HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABE2033-25UG 04065268364581