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CP28 Anti-Fibroblast Antigen Mouse mAb (AS02)

Anti-Fibroblast Antigen, mouse monoclonal, clone AS02, detects ~30-31 kDa CD90/Thy-1 antigen in human fibroblasts (non-reducing conditions). It is validated for FC, WB, IF, IP & IHC (frozen sections).

Anti-Fibroblast Antigen Mouse mAb (AS02) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-CD90 (Ab-1) (Mouse), Anti-Thy-1 (Ab-1) (Mouse)

CP28
  
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Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Species ReactivityHostAntibody Type
H, MkMMonoclonal Antibody
Description
OverviewRecognizes the ~30-31 kDa CD90/Thy-1 antigen in human fibroblasts under non-reducing conditions.
Catalogue NumberCP28
Brand Family Calbiochem®
SynonymsAnti-CD90 (Ab-1) (Mouse), Anti-Thy-1 (Ab-1) (Mouse)
References
ReferencesSaalbach, A., et al. 1996 J. Invest. Dermatol. 106, 1314.
Janin, A., et al. 1990. Clin. Exp. Rheum. 8, 237.
Kay, M., et al. 1989. J. Invest. Dermatol. 92, 168.
Esterre, P.H., et al. 1984 Cell Mol. Biol. 38, 297.
Hoyhtya, M., et al. 1984 Eur. J. Biochem 141, 477.
Product Information
DeclarationManufactured by Dianova, GmbH, Germany.
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 6% BSA.
Positive controlAny fibroblast cell line or skin tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesSaalbach, A., et al. 2000. J. Invest. Dermatol. 115, 882. Saalbach, A., et al. 1999. Cell Tissue Res. 298, 307. Saalbach, A., et al. 1998. Arch. Dermatol. Res. 290, 360. Saalbach, A., et al. 1997. Cell Tissue Res. 290, 539. Saalbach, A., et al. 1996. J. Invest. Dermatol. 106, 1314.
Key Applications Flow Cytometry
Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Application NotesFlow Cytometry (see comments)
Frozen Sections (10 µg/ml, acetone fixation)
Immunoblotting (10 µg/ml, non-reducing only)
Immunofluorescence (10 µg/ml)
Immunoprecipitation (2-10 µg/ml)
Application CommentsThis antibody has been used with magnetic beads to remove fibroblasts from mixed cultures. It is also suitable for flow cytometry studies (see application references). Note that in healthy tissue (skin, placenta, liver, heart), this antibody does not react with endothelial cells. However, endothelial cells activated by PMA, TNFα, or IFNγ may be detected by this antibody, suggesting activated endothelial cells may express CD90 and that CD90 may be an activation marker of human endothelial cells. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenhuman fibroblasts
ImmunogenHuman
CloneAS02
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Monkey
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
CP28 0

Documentation

Anti-Fibroblast Antigen Mouse mAb (AS02) MSDS

Title

Safety Data Sheet (SDS) 

Anti-Fibroblast Antigen Mouse mAb (AS02) Certificates of Analysis

TitleLot Number
CP28

References

Hivatkozások áttekintése
Saalbach, A., et al. 1996 J. Invest. Dermatol. 106, 1314.
Janin, A., et al. 1990. Clin. Exp. Rheum. 8, 237.
Kay, M., et al. 1989. J. Invest. Dermatol. 92, 168.
Esterre, P.H., et al. 1984 Cell Mol. Biol. 38, 297.
Hoyhtya, M., et al. 1984 Eur. J. Biochem 141, 477.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision21-August-2007 RFH
SynonymsAnti-CD90 (Ab-1) (Mouse), Anti-Thy-1 (Ab-1) (Mouse)
ApplicationFlow Cytometry (see comments)
Frozen Sections (10 µg/ml, acetone fixation)
Immunoblotting (10 µg/ml, non-reducing only)
Immunofluorescence (10 µg/ml)
Immunoprecipitation (2-10 µg/ml)
DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with X63-Ag.8.653 mouse myeloma cells. Recognizes the ~30-31 kDa fibroblast antigen (CD90/Thy-1) under non-reducing conditions.
BackgroundThe ability to distinguish fibroblast contamination in cell cutures and tissues is an important biological problem. Identification of fibroblastic components of cells and tissues is also useful to determine the potential fibroblastic origin of tumors. The ability to specifically detect fibroblasts in tissue culture samples and in tissue samples in situ is often difficult. One of the chief problems has been the identification of a protein which is specific to fibroblasts and not shared by other cell types such as endothelial cells and chondrocytes. Most fibroblast specific reagents show some cross reactivity with monocytes/macrophages and smooth muscle cells. Currently, the most commonly used approach has been to use antibodies to proline-4-hydroxylase, a protein involved in the synthesis of collagen. However, proline-4-hydroxylase is present in all cells which make collagen, not just fibroblasts. Also, proline-4-hydroxylase is intracellular, which makes it less attractive to many as a marker, and most antibodies to proline-4-hydroxylase will not stain quiescent cells that are not actively producing collagen. CD90, or Thy-1 surface antigen, is restricted to the expression on extracellular membrane of fibroblast cells, neurons, and some CD34+ blood stem cells. This antibody shows no reactivity with keratinocytes, chondrocytes, smooth muscle, Langerhans, or endothelial cells in situ nor will it bind to extracellular matrix proteins. The antigen shows no change in levels of expression under different growth conditions including changes in serum levels, passage number of cells, etc. Interestingly, the level of CD90 expression increases in tissues during wound healing.
HostMouse
Immunogen speciesHuman
Immunogenhuman fibroblasts
CloneAS02
IsotypeIgG₁
Specieshuman, monkey, not mouse, not rat
Positive controlAny fibroblast cell line or skin tissue
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 6% BSA.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThis antibody has been used with magnetic beads to remove fibroblasts from mixed cultures. It is also suitable for flow cytometry studies (see application references). Note that in healthy tissue (skin, placenta, liver, heart), this antibody does not react with endothelial cells. However, endothelial cells activated by PMA, TNFα, or IFNγ may be detected by this antibody, suggesting activated endothelial cells may express CD90 and that CD90 may be an activation marker of human endothelial cells. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Toxicity Standard Handling
ReferencesSaalbach, A., et al. 1996 J. Invest. Dermatol. 106, 1314.
Janin, A., et al. 1990. Clin. Exp. Rheum. 8, 237.
Kay, M., et al. 1989. J. Invest. Dermatol. 92, 168.
Esterre, P.H., et al. 1984 Cell Mol. Biol. 38, 297.
Hoyhtya, M., et al. 1984 Eur. J. Biochem 141, 477.
Application referencesSaalbach, A., et al. 2000. J. Invest. Dermatol. 115, 882. Saalbach, A., et al. 1999. Cell Tissue Res. 298, 307. Saalbach, A., et al. 1998. Arch. Dermatol. Res. 290, 360. Saalbach, A., et al. 1997. Cell Tissue Res. 290, 539. Saalbach, A., et al. 1996. J. Invest. Dermatol. 106, 1314.