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ABS2124 Anti-Ferrochelatase

Anti-Ferrochelatase, Cat. No. ABS2124, is a rabbit polyclonal antibody that detects Ferrochelatase, mitochondrial. It has been tested for use in Immunoprecipitation and Western Blotting.

Anti-Ferrochelatase MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: FECH UniProt Number: P22830
ABS2124
100 µL  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HWB, IPRbSerumPolyclonal Antibody
Description
Catalogue NumberABS2124
DescriptionAnti-Ferrochelatase
Alternate Names
  • Heme synthase
  • protoheme ferro-lyase
Background InformationFerrochelatase, mitochondrial (UniProt: P22830; also known as EC: 4.99.1.1, Heme synthase, Protoheme ferro-lyase) is encoded by the FECH gene (Gene ID: 2235) in human. Ferrochelatase catalyzes the terminal reaction of the heme biosynthetic pathway in all cells. It catalyzes the insertion of ferrous iron into protoporphyrin IX, yielding heme. In eukaryotic cells, it is located in the inner mitochondrial membrane with its active site facing the mitochondrial matrix. Human ferrochelatase is a homodimer with each subunit composed of five regions: a mitochondrial localization sequence, the N terminal domain, two folded domains, and a C terminal extension. The active pocket of ferrochelatase consists of two hydrophobic "lips" and a hydrophilic interior. The hydrophobic lips face the inner mitochondrial membrane and facilitate the passage of the poorly soluble protoporphyrin IX substrate and the heme product via the membrane. Reduced levels of ferrochelatase activity are observed in patients with protoporphyria. Point-mutations in the FECH gene are reported to be associated with certain cases of erythropoietic protoporphyria.
References
Product Information
FormatSerum
PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Ferrochelatase, Cat. No. ABS2124, is a rabbit polyclonal antibody that detects Ferrochelatase, mitochondrial. It has been tested for use in Immunoprecipitation and Western Blotting.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesImmunoprecipitation Analysis: A representative lot detected Ferrochelatase in differentiated mouse erythroleukemia (MEL) cells (Karr, S.R., et. al. (1988). Biochem J. 254(3):799-803).

Western Blotting Analysis: A representative lot detected Ferrochelatase in differentiated mouse erythroleukemia (MEL) cells and transfected heterologous cells. (Chen, W., et. al. (2010). Blood. 116(4):628-30; Anand, S., et. al. (2009). Clin Cancer Res. 15(10):3333-43; Medlock, A.E., et. al. (2015). PLoS One. 10(8):e0135896).
Biological Information
ImmunogenFlag-tagged recombinant fragment corresponding to the mature mitochondrial form containing 369 amino acids (aa 55-423) from human Ferrochelatase.
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody detects ferrochelatase in human and murine species. It targets the mature form of ferrochelatase.
IsotypeIgG
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • FECH
Purification MethodUnpurified
UniProt Number
Molecular Weight~43 kDa observed; 47.86 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Ferrochelatase in 10 µg of K562 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
ABS2124 04054839223600