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ST1137 Anti-FOXP3 Mouse mAb (3G3)

Anti-FOXP3 Mouse mAb (3G3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
ST1137
  
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Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~47 kDa FOXP3 protein in lymph node cells.
Catalogue NumberST1137
Brand Family Calbiochem®
Application Data
Detection of human FOXP3 by immunoblotting. Samples: whole cell lysate from HEK293 cells transfected with full-length FOXP3 (lane 1) or mock-transfected (lane 2). Primary antibody: Anti-FOXP3 Mouse mAb (3G3) (Cat. No. ST1137) (2 µg/ml). Detection: chemiluminescence.

Detection of human FOXP3 by flow cytometry. Samples: mouse lymph node cells (2x106 cells). Primary antibodies: Anti-FOXP3 Mouse mAb (3G3) (Cat. No. ST1137) (100 ng in 50 µl total volume) (upper panel) and Anti-CD4 control antibody (lower panel). Detection: fluorescence.
References
ReferencesGavin, M.A., et al. 2006.Proc Natl Acad Sci USA 17, 6659.
Product Information
FormLiquid
FormulationIn PBS, 0.05% BSA.
Positive controlLymph node cells
Preservative≤ 0.1% sodium azide
Applications
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Application NotesImmunoblotting (2 µg/ml)
Flow cytometry (100 ng/2 x 10⁶ cells in 50 µl total volume)
Application CommentsAntibody should be titrated for optimal results in individual sytems.
Biological Information
Immunogenfull-length, recombinant, mouse FOXP3 containing a His•Tag® sequence
ImmunogenMurine
EpitopeWithin the N-terminus
Clone3G3
HostMouse
IsotypeIgG1
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
ST1137 0

Documentation

References

Hivatkozások áttekintése
Gavin, M.A., et al. 2006.Proc Natl Acad Sci USA 17, 6659.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision28-July-2008 RFH
ApplicationImmunoblotting (2 µg/ml)
Flow cytometry (100 ng/2 x 10⁶ cells in 50 µl total volume)
Application Data
Detection of human FOXP3 by immunoblotting. Samples: whole cell lysate from HEK293 cells transfected with full-length FOXP3 (lane 1) or mock-transfected (lane 2). Primary antibody: Anti-FOXP3 Mouse mAb (3G3) (Cat. No. ST1137) (2 µg/ml). Detection: chemiluminescence.

Detection of human FOXP3 by flow cytometry. Samples: mouse lymph node cells (2x106 cells). Primary antibodies: Anti-FOXP3 Mouse mAb (3G3) (Cat. No. ST1137) (100 ng in 50 µl total volume) (upper panel) and Anti-CD4 control antibody (lower panel). Detection: fluorescence.
DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~47 kDa FOXP3 protein.
BackgroundOne of the many immunotolerance mechanisms that the immune system has developed to distinguish between self and non-self antigens is regulatory T cells. Several experiments using transgenic mice and retrovirus-mediated overexpression studies, have led to the identification of FOXP3, a transcription factor, as a specific molecular marker essential for the development and function of regulatory T cells. The primary evidence regarding the involvement of FOXP3 in the development of these cells was provided in patients suffering from IPEX, a rare and fatal human autoimmune disease. The emergence of regulatory T cells and the role of FOXP3 as a critical player in the negative control of various normal and pathological immune responses holds great promise for the development of novel therapies for autoimmune diseases.
HostMouse
Immunogen speciesMurine
Immunogenfull-length, recombinant, mouse FOXP3 containing a His•Tag® sequence
EpitopeWithin the N-terminus
Clone3G3
IsotypeIgG1
Specieshuman, mouse
Positive controlLymph node cells
FormLiquid
FormulationIn PBS, 0.05% BSA.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤ 0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual sytems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesGavin, M.A., et al. 2006.Proc Natl Acad Sci USA 17, 6659.