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ABD71 Anti-FOXC1 Antibody

Anti-FOXC1 Antibody is a Rabbit Polyclonal Antibody for detection of FOXC1 also known as Forkhead box protein C1, Forkhead-related protein FKHL7, FREAC-3 & has been validated in WB, ICC.

Anti-FOXC1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: FOXC1, FKHL7, FREAC3 UniProt Number: Q12948
ABD71
100 µg  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABD71
DescriptionAnti-FOXC1 Antibody
Alternate Names
  • Forkhead box protein C1
  • Forkhead-related protein FKHL7
  • Forkhead-related transcription factor 3
  • FREAC-3
Background InformationForkhead box protein C1 (FOXC1) is a member of the family of forkhead transcription factors. These proteins are characterized by the forkhead domain--a 110 amino acid domain that binds to DNA molecules. FOXC1 has been implicated in the development of the eye; disruption of the FOXC1 gene has been linked to a number of glaucoma-related diseases such as Axenfeld-Rieger syndrome.
References
Product Information
FormatAffinity Purified
Control
  • THP cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-FOXC1 Antibody is a Rabbit Polyclonal Antibody for detection of FOXC1 also known as Forkhead box protein C1, Forkhead-related protein FKHL7, FREAC-3 & has been validated in WB, ICC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blot Analysis: A representative lot of this antibody detected FOXC1 in HeLa, HEK293, THP1, and MDA-MB-468 cell lysates, and in human bone marrow tissue lysate.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected FOXC1 in HeLa cells. This antibody positively stains the nucleus, with some cytoplasm staining. This staining pattern has also been observed by an independent laboratory (Berry, F. B., et al. (2002). J Biol Chem. 277(12):10292-10297.).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human FOXC1.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Mouse based on 100% sequence homology. Other homologies: Rat (71% sequence homology).
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it has been shown to play a role in the regulation of embryonic and ocular development. Mutations in this gene cause various glaucoma phenotypes including primary congenital glaucoma, autosomal dominant iridogoniodysgenesis anomaly, and Axenfeld-Rieger anomaly.
Gene Symbol
  • FOXC1
  • FKHL7
  • FREAC3
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Binding of FREAC-3 and FREAC-4 to their cognate sites results in bending of the DNA at an angle of 80-90 degrees.

SUBUNIT STRUCTURE: Monomer.

SUBCELLULAR LOCATION: Nucleus.

TISSUE SPECIFICITY: Expressed in all tissues and cell lines examined.

INVOLVEMENT IN DISEASE: Defects in FOXC1 are the cause of Axenfeld-Rieger syndrome type 3 (RIEG3); also known as Axenfeld-Rieger syndrome (ARS) or Axenfeld syndrome or Axenfeld anomaly. It is characterized by posterior corneal embryotoxon, prominent Schwalbe line and iris adhesion to the Schwalbe line. Other features may be hypertelorism (wide spacing of the eyes), hypoplasia of the malar bones, congenital absence of some teeth and mental retardation. When associated with tooth anomalies, the disorder is known as Rieger syndrome. Glaucoma is a progressive blinding condition that occurs in approximately half of patients with Axenfeld-Rieger malformations. Defects in FOXC1 are the cause of iridogoniodysgenesis anomaly (IGDA). IGDA is an autosomal dominant phenotype characterized by iris hypoplasia, goniodysgenesis, and juvenile glaucoma. Defects in FOXC1 are a cause of Peters anomaly (PAN). Peters anomaly consists of a central corneal leukoma, absence of the posterior corneal stroma and Descemet membrane, and a variable degree of iris and lenticular attachments to the central aspect of the posterior cornea.

SEQUENCE SIMILARITIES: Contains 1 fork-head DNA-binding domain.
Molecular Weight~75 kDa observed. Uniprot describes a molecular weight at ~57 kDa. This protein may be observed at ~65 kDa in some cell lysates. (Tamimi, Y., et al. (2006). Hum Mol Genet. 15(21): 3229-3240.).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in THP1 cell lysate.

Western Blot Analysis: 0.025 µg/mL of this antibody detected FOXC1 in 10 µg of THP1 cell lysate. No Tween® reagent was used in the wash buffers and in the primary and secondary antibody dilution buffers.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
ABD71 04053252691171

Documentation

Anti-FOXC1 Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-FOXC1 Antibody Certificates of Analysis

TitleLot Number
Anti-FOXC1 - 2246785 2246785
Anti-FOXC1 - 3191457 3191457
Anti-FOXC1 - 3684751 3684751
Anti-FOXC1 - 3891813 3891813
Anti-FOXC1 - Q2005666 Q2005666
Anti-FOXC1 -2767005 2767005
Anti-FOXC1 -2774074 2774074
Anti-FOXC1 Polyclonal Antibody 2950903
Anti-FOXC1 Polyclonal Antibody 2935442
Anti-FOXC1 Polyclonal Antibody 3051278
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Brochure

Title
New Products: Volume 3, 2012