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MABC1748-100UL Anti-FIP200 Antibody, clone N1-8

Anti-FIP200, clone N1-8, Cat. No. MABC1748, is a mouse monoclonal antibody that detects FAK family kinase-interacting protein of 200 kDa (FIP200) and is tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.

MABC1748-100UL
100 µL  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue NumberMABC1748-100UL
DescriptionAnti-FIP200 Antibody, clone N1-8
Alternate Names
  • RB1-inducible coiled-coil protein 1
  • FAK family kinase-interacting protein of 200 kDa
Background InformationRB1-inducible coiled-coil protein 1 (UniProt: Q8TDY2; also known as FAK family kinase-interacting protein of 200 kDa, FIP200) is encoded by the RB1CC1 (also known as KIAA0203; RBICC) gene (Gene ID: 9821) in human. FIP200 plays an important role in several biological pathways including cell proliferation and autophagy. It regulates early and late events of autophagosome formation through direct interaction with Atg16L1. FIP200 expression has been shown in cytoplasm and in the nucleus. It serves as a positive regulator of the NF-kB and mTOR signaling. It also functions as a negative factor in FAK-Erk1/2 signaling pathway. It is reported to form a complex with p53 and/or hSNF5 and the large protein complex in mammalian cells and serves as a strong activator of retinoblastoma 1 (RB1), p16, and p21 promoters. The nuclear FIP200 is shown to enhance the RB1 pathway globally and behave as a tumor suppressor in some human malignancies. Its expression levels correlate well with those of RB1 in cancer cell lines and higher expression has been shown in human musculoskeletal and cultured osteosarcoma cells. Clone N1-8 is highly suited for immunohistochemistry analysis and it recognizes an epitope within amino acids 255-271. (Ref.: Hama, Y., et al. (2012). PLoS ONE 7(3); e32052).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-FIP200, clone N1-8, Cat. No. MABC1748, is a mouse monoclonal antibody that detects FAK family kinase-interacting protein of 200 kDa (FIP200) and is tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • ELISA
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesELISA Analysis: A representative lot detected FIP200 in ELISA applications (Hama, Y., et. al. (2012). PLoS One. 7(3):e32052).

Immunohistochemistry Applications: A representative lot detected FIP200 in Immunohistochemistry applications (Hama, Y., et. al. (2012). PLoS One. 7(3):e32052).

Western Blotting Analysis: A representative lot detected FIP200 in Western Blotting applications (Hama, Y., et. al. (2012). PLoS One. 7(3):e32052).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to 247 amino acids from the N-terminal region of human FIP200 and a C-terminal polypeptide fragment of FIP200.
EpitopeN-terminus
CloneN1-8
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone N1-8 is a mouse monoclonal antibody that detects Focal adhesion kinase (FAK)-interacting protein of 200 kDa (FIP200). It targets an epitope within 17 amino acids from the N-terminal half.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • RB1CC1
  • KIAA0203
  • RBICC
Purification MethodProtein G purified
UniProt Number
Molecular Weight183.09 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human breast cancer tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected FIP200 in human breast cancer tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABC1748-100UL 04061842667628

Documentation

Anti-FIP200 Antibody, clone N1-8 MSDS

Title

Safety Data Sheet (SDS) 

Anti-FIP200 Antibody, clone N1-8 Certificates of Analysis

TitleLot Number
Anti-FIP200, clone N1-8 - Q3509822 Q3509822

References

Reference overviewPub Med ID
Preparation of mouse monoclonal antibody for RB1CC1 and its clinical application
Yusuke Hama 1 , Tokuhiro Chano, Takuma Inui, Kyoichi Matsumoto, Hidetoshi Okabe
PLos One  7(3)  e32052  2011

Kivonat megmutatása 		22396748 22396748