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07-172-I Anti-FHIT

Anti-FHIT, Cat. No. 07-172-I, is a highly specific rabbit polyclonal antibody that targets FHIT and has been tested in Immunoprecipitation and Western Blotting.

Anti-FHIT MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: FHIT UniProt Number: P49789
07-172-I
100 μg  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IPRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-172-I
Replaces07-172
DescriptionAnti-FHIT
Alternate Names
  • Bis(5'-adenosyl)-triphosphatase
  • AP3A hydrolase
  • AP3Aase, Diadenosine 5',5'''-P1,P3-triphosphate hydrolase
  • Dinucleosidetriphosphatase
  • Fragile histidine triad protein
Background InformationBis(5'-adenosyl)-triphosphatase (EC 3.6.1.29; UniProt P49789; also known as AP3A hydrolase, AP3Aase, Diadenosine 5',5'''-P1,P3-triphosphate hydrolase, Dinucleosidetriphosphatase, Fragile histidine triad protein) is encoded by the FHIT gene (Gene ID 2722) in human. Common fragile sites (CFSs) are large genomic loci that display chromosomal instability in response to genotoxic or replicative stress. Many CFSs harbor genes that contribute to cancer development and are non-randomly altered in cancers through genomic alteration. The Fragile histidine triad (FHIT) gene at FRA3B is one of the earliest and most frequently altered genes in the majority of human cancers. FHIT protein plays a role in apoptosis and prevention of the epithelial-mesenchymal transition (EMT). A FHIT-upregulated microRNA, miR-30c, has been identified to mediate the suppression of EMT and metastasis by directly targeting metastasis genes Metadherin (MTDH), High-mobility group AT-hook 2 (HMGA2), and the mesenchymal markers, Vimentin (VIM) and Fibronectin (FN1), in human lung cancer. In addition, FHIT protein also plays an important role in maintaining genome stability, loss of FHIT protein function leads to nucleotide imbalance, spontaneous replication stress, and DNA breaks. Because FHIT loss-induced DNA damage is checkpoint-independent, cells accumulate further DNA damage during subsequent cell cycles, accruing global genome instability that could facilitate oncogenic mutation acquisition and expedite clonal expansion.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-FHIT, Cat. No. 07-172-I, is a highly specific rabbit polyclonal antibody that targets FHIT and has been tested in Immunoprecipitation and Western Blotting.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesImmunoprecipitation Analysis: 5 µg from a representative lot immunoprecipited FHIT from 500 µg of A549 cell lysate.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to a C-terminal region sequence of human FHIT.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody targets a C-terminal epitope region outside the HIT domain.
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • FHIT
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~17 kDa observed. 16.86 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected FHIT in 10 µg of A549 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
07-172-I 04054839069116