Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABN807 Anti-EYS Antibody, clone 3G10.1

Anti-EYS Antibody, clone 3G10.1 is an antibody against EYS for use in Western Blotting, Immunohistochemistry (Paraffin).

Anti-EYS Antibody, clone 3G10.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Sensory & PNS Gene Symbol: EYS, C6orf178, C6orf179, C6orf180, EGFL10, EGFL11, SPAM, UNQ9424/PRO34591 UniProt Number: Q5T1H1
MABN807
100 μg  
Purchase on Sigma-Aldrich

Különleges ajánlatok

Áttekintés

Replacement Information

Különleges ajánlatok

100% Performance Guaranteed

Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN807
DescriptionAnti-EYS Antibody, clone 3G10.1
Alternate Names
  • Protein eyes shut homolog
  • EGF-like protein 10
  • EGF-like protein 11
  • Epidermal growth factor-like protein 10
  • Epidermal growth factor-like protein 11
  • Protein spacemaker homolog
Background InformationProtein eyes shut homolog (UniProt Q5T1H1; also known as EGF-like protein 10, EGF-like protein 11, Epidermal growth factor-like protein 10, Epidermal growth factor-like protein 11, Protein spacemaker homolog) is encoded by the EYS (also known as C6orf178, C6orf179, C6orf180, EGFL10, EGFL11, SPAM) gene (ORF UNQ9424/PRO34591, Gene ID 346007) in human. Drosophila eyes shut protein is expressed in photoreceptor cells, the central nervous system, and peripheral sensory ganglia, and is essential for the formation of a matrix-filled intra-rhabdomeral space within the fly's retina ommatidia, the loss of spacemaker (spam) converts an open rhabdom system to a closed one. Human EYS/SPAM gene likewise is found to be expressed in the eye and the spinal cord, and mutations in human EYS result in retinitis pigmentosa. Human EYS protein contains 27 EGF-like repeats and five laminin G-like (LG1-5) domains (between EGF repeats 21 & 21, 21 & 22, 23 & 24, 25 & 26, and after repeat EGF repeat 27). The presence of the EGF-like repeats is indicative of a role in the Notch signaling pathway, while EYS LG1-5 bear resemblance to the three agrin laminin G domains that are known to interact with several proteins, including α-dystroglycan at the muscle surface.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-EYS Antibody, clone 3G10.1 is an antibody against EYS for use in Western Blotting, Immunohistochemistry (Paraffin).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected EYS in human retina tissue sections.
Biological Information
ImmunogenGST-tagged recombinant human EYS fragment corresponding to the EGF-like domains 9-11.
EpitopeEGF-like domains 9-11.
Clone3G10.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityExpected to react with spliced isoforms 1 and 3, but not isoforms 2 and 4, reported by UniProt (Q5T1H1).
IsotypeIgG2bκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • EYS
  • C6orf178
  • C6orf179
  • C6orf180
  • EGFL10
  • EGFL11
  • SPAM
  • UNQ9424/PRO34591
Purification MethodProtein G Purified
UniProt Number
Molecular Weight348.4 kDa (isoform 1) and 350.8 kDa (isoform 3) kDa calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting of EYS recombinant protein.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected EYS recombinant protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABN807 04055977333602