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DR1079 Anti-ERCC1 Mouse mAb (3A7)

Anti-ERCC1 Mouse mAb (3A7) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Excision Repair Cross-Complementing 1

DR1079
  
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Replacement Information

Kulcsspecifikációk táblázata

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M
Description
Overview

This product has been discontinued.





Recognizes the ERCC1 protein.
Catalogue NumberDR1079
Brand Family Calbiochem®
SynonymsAnti-Excision Repair Cross-Complementing 1
Application Data
Detection of human ERCC1 by immunoblotting. Sample: Partial recombinant protein. Primary antibody: Anti-ERCC1 Mouse mAb (3A7) (Cat. No. DR1079) (1-5 µg/ml). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids of 207-282 human ERCC1, expressed as a GST fusion protein
Clone3A7
HostMouse
IsotypeIgG2a
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
DR1079 0

Documentation

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-November-2010 RFH
SynonymsAnti-Excision Repair Cross-Complementing 1
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human ERCC1 by immunoblotting. Sample: Partial recombinant protein. Primary antibody: Anti-ERCC1 Mouse mAb (3A7) (Cat. No. DR1079) (1-5 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ERCC1 protein.
BackgroundERCC1 functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light. This protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids of 207-282 human ERCC1, expressed as a GST fusion protein
Clone3A7
IsotypeIgG2a
Specieshuman
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review