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MABS2238-100UG Anti-ELOVL5 Antibody, clone Z88

Anti-ELOVL5 clone Z88, Cat. No. MABS2238, is a mouse monoclonal antibody that detects ELOVL5 and is tested for use in ELISA, Immunohistochemistry, and Western Blotting.

MABS2238-100UG
100 μg  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue NumberMABS2238-100UG
DescriptionAnti-ELOVL5 Antibody, clone Z88
Alternate Names
  • Elongation of very long chain fatty acids protein 5
  • EC:2.3.1.199
  • 3-keto acyl-CoA synthase ELOVL5
  • ELOVL fatty acid elongase 5
  • ELOVL FA elongase 5
  • Fatty acid elongase 1
  • hELO1
  • Very long chain 3-ketoacyl-CoA synthase 5
  • Very long chain 3-oxoacyl-CoA synthase 5
Background InformationElongation of very long chain fatty acids protein 5 (UniProt: EC:2.3.1.199, 3-keto acyl-CoA synthase ELOVL5, ELOVL fatty acid elongase 5, ELOVL FA elongase 5, Fatty acid elongase 1, hELO1, Very long chain 3-ketoacyl-CoA synthase 5, Very long chain 3-oxoacyl-CoA synthase 5) is encoded by the ELOVL5 (also known as EVOVL2, PRO0530) gene (Gene ID: 60481) in human. ELOVL5 is a widely expressed, multi-pass membrane protein found in the endoplasmic reticulum (ER). Higher expression of ELOVL5 is reported in the adrenal gland and testis. It catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. It exhibits marked preference for polyunsaturated molecules. It allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Significantly higher levels of ELOVL5 are reported in prostate cancer compared to nonmalignant prostate. ELOVL5 depletion is reported to markedly alter mitochondrial morphology and function that leads to excess generation of reactive oxygen species and results in suppression of prostate cancer cell proliferation, in vivo tumor growth, and metastasis. ELOVL5 is expressed in the brain in a region- and cell type-specific manner and is abundant in the cerebellum, brainstem, and primary and accessory olfactory regions. Mutations of ELOVL5 gene are known to cause the spino-cerebellar ataxia type 38 (SCA38), a rare autosomal neurological disease characterized by gait abnormality, dysarthria, dysphagia, hyposmia, and peripheral neuropathy. (Ref.: Balbo, I., et al. (2021). Front. Neuroanat. 15; 669073; Centenera, MM., et al. (2021). Cancer Res. 81(7); 1704-1718; Alnabulsi, A., et al. (2019). Int. J. Cancer. 145(4);1138-1147).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-ELOVL5 clone Z88, Cat. No. MABS2238, is a mouse monoclonal antibody that detects ELOVL5 and is tested for use in ELISA, Immunohistochemistry, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunohistochemistry (Paraffin)
  • ELISA
Application NotesTested Applications

Western Blotting Analysis: A representative lot detected ELOVL5 in Western Blotting applications (Alnabulsi, A., et al. (2019). Int J Cancer. 145(4):1138-1147).

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ELOVL5 in human prostate tissue sections.

ELISA Analysis: A representative lot detected ELOVL5 in ELISA applications (Alnabulsi, A., et al. (2019). Int J Cancer. 145(4):1138-1147).

Immunohistochemistry Applications: A representative lot detected ELOVL5 in Immunohistochemistry applications (Alnabulsi, A., et al. (2019). Int J Cancer. 145(4):1138-1147).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenOvalbumin-conjugated linear peptide corresponding to 9 amino acids from the C-terminal region of human Elongation of very long chain fatty acids protein 5 (ELOVL5).
EpitopeC-terminal
CloneZ88
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone Z88 is a mouse monoclonal antibody that detects Elongation of very long chain fatty acids protein 5 (ELOVL5). It targets an epitope within 9 amino acids from the C-terminal region.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Monkey based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ELOVL5
Purification MethodPurified
UniProt Number
Molecular Weight~38 kDa observed; 35.29 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in MCF-7 cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ELOVL5 in MCF-7 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABS2238-100UG 04065268042038