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AB5976 Anti-Dishevelled-2 Antibody

This Anti-Dishevelled-2 Antibody is validated for use in WB for the detection of Dishevelled-2.

Anti-Dishevelled-2 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Hematopoietic Stem Cells, Neural Stem Cells Gene Symbol: DVL2, Dishevelled-2 UniProt Number: O14641
AB5976
50 µL  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberAB5976
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Dishevelled-2 Antibody
Alternate Names
  • DVL2
  • DSH Homolog 2
Background InformationDVL2 is a member of multigene family that also contains DVL1 and DVL3. The vertebrate dishevelled proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. DVL2 encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein.
References
Product Information
FormatPurified
PresentationPartially purified immunoglobulin. Liquid in PBS, pH 7.4, containing 0.01M sodium azide.
Quality LevelMQ300
Applications
ApplicationThis Anti-Dishevelled-2 Antibody is validated for use in WB for the detection of Dishevelled-2.
Key Applications
  • Western Blotting
Application NotesWestern blot: 1:1,000 using ECL. The antibody reacts with a protein of ~95kDa.

Dilute antibody in 50 mM PBS, pH 7.4 containing 1.5% sodium chloride and 1% normal animal serum (same species as the host of the secondary antibody).

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenSynthetic peptide corresponding to amino acids 633-645 of human disheveiled-2 protein.
HostRabbit
SpecificityDishevelled-2 protein (dvl-2). By Western blot the antibody shows no cross reactivity with recombinant dishevelled-1 (dvl-1) or dishevelled-3 (dvl-3) protein. Pre-adsorption of the antibody with the immunogen peptide completely abolishes its reactivity by Western blot against disheveiled-2 protein.
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the dishevelled (dsh) protein family. The vertebrate dsh proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. This gene encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein, which may play a role in the signal transduction pathway mediated by multiple Wnt proteins. The mechanisms of dishevelled function in Wnt signaling are likely to be conserved among metazoans.
Gene Symbol
  • DVL2
  • Dishevelled-2
UniProt Number
UniProt SummaryFUNCTION: SwissProt: O14641 # May play a role in the signal transduction pathway mediated by multiple Wnt genes.
SIZE: 736 amino acids; 78948 Da
SUBUNIT: Interacts through its PDZ domain with the C-terminal regions of VANGL1 and VANGL2 (By similarity). Interacts with DIXDC1 and Rac.
SUBCELLULAR LOCATION: Cytoplasm.
PTM: Phosphorylated.
SIMILARITY: SwissProt: O14641 ## Belongs to the DSH family. & Contains 1 DEP domain. & Contains 1 DIX domain. & Contains 1 PDZ (DHR) domain.
Stem Cell Type
  • Neural Stem Cells
  • Hematopoietic Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted aliquots (not less than 10 μL) for up to 6 months. Avoid repeated freeze/thaw cycles. The use of high quality antiserum-grade plastic or glass is recommended.
Packaging Information
Material Size50 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
AB5976 04053252731532