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MAB3234 Anti-Cytokeratin 18 Antibody, clone RGE53

MAB3234
100 µg  
Purchase on Sigma-Aldrich

Különleges ajánlatok

Áttekintés

Replacement Information

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Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
Ca, Ch, H, Ht, M, Po, R, RbFC, ICC, IHC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB3234
Replaces04-586
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Cytokeratin 18 Antibody, clone RGE53
Background InformationCytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight from between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8, The individual cytokeratin polypeptides are designated 1-20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.
References
Product Information
FormatPurified
HS Code3002 15 90
Control
  • Skin , Pancreas/tumor
PresentationProtein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Quality LevelMQ100
Applications
ApplicationAnti-Cytokeratin 18 Antibody, clone RGE53 is an antibody against Cytokeratin 18 for use in FC, IC, IH & WB.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blotting
Applications Not Recommended
  • Immunohistochemistry (Paraffin)
Application NotesWestern blot

Immunohistochemistry on fresh frozen or acetone fixed tissue sections. Formalin fixed, paraffin embedded tissues are not recommended for RGE53.

Immunocytochemistry: (10μg/mL) Light fixation with 4% formalin, 2.5% PFA ( 5-10 minutes room temperature) or acetone. {See, Barrett, et al 2001} Permeabilization with 0.2% triton X-100 in blocking buffer if using formalin or PFA fixations.

Flow cytometry: Acetone fixed/ethanol fixed cells {See Mezosi, E et al, 2002}.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenCytoskeletal preparation from HeLa cells
CloneRGE53
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificitySpecifically recognizes glandular epithelial cells from gastro-intestinal tract, respiratory tract and urogenetical tract. As well as endocrine and exocrine tissues and mesothelial cells. It is unreactive with stratified squamous epithelia (esophagus, epidermis, ectocervix). In transitional epithelium of the bladder RGE53 only reacts with the superficial (umbrella) cells. RGE53 can distinguish adenocarcinomas and mesotheliomas from squamous cell carcinoma and non-epithelial tumours. The antibody is directed against 45 kD cytokeratin protein (cytokeratin 18). Cross reactivity with; Keratin from most mammals, birds.
IsotypeIgG1
Species Reactivity
  • Canine
  • Chicken
  • Human
  • Hamster
  • Mouse
  • Pig
  • Rat
  • Rabbit
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryKRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
Gene Symbol
  • KRT18
  • K18
  • Keratin-18
  • CYK18
  • Cytokeratin-18
  • CK-18
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P05783 # Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
SIZE: 430 amino acids; 48058 Da
SUBUNIT: Heterotetramer of two type I and two type II keratins. Keratin-18 associates with keratin-8. Interacts with the thrombin- antithrombin complex (By similarity). Interacts with PNN, HCV core protein and mutated CFTR. Interacts with YWHAE, YWHAH and YWHAZ only when phosphorylated. Interacts with DNAJB6, TCHP and TRADD.
SUBCELLULAR LOCATION: Cytoplasm, perinuclear region.
TISSUE SPECIFICITY: Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
PTM: Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. & Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.DISEASE:SwissProt: P05783 # Defects in KRT18 are a cause of cryptogenic cirrhosis [MIM:215600].
SIMILARITY: SwissProt: P05783 ## Belongs to the intermediate filament family.
MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
Molecular Weight45 kD
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MAB3234 04053252667343

Documentation

Anti-Cytokeratin 18 Antibody, clone RGE53 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Cytokeratin 18 Antibody, clone RGE53 Certificates of Analysis

TitleLot Number
MOUSE ANTI-CYTOKERATIN 18 3169506
MOUSE ANTI-CYTOKERATIN 18 MONOCLONAL ANTIBODY - 2390416 2390416
MOUSE ANTI-CYTOKERATIN 18 - 2557570 2557570
MOUSE ANTI-CYTOKERATIN 18 - 3191428 3191428
MOUSE ANTI-CYTOKERATIN 18 - 3466664 3466664
MOUSE ANTI-CYTOKERATIN 18 - 3508422 3508422
MOUSE ANTI-CYTOKERATIN 18 - 3594318 3594318
MOUSE ANTI-CYTOKERATIN 18 - 3703709 3703709
MOUSE ANTI-CYTOKERATIN 18 - 3849157 3849157
MOUSE ANTI-CYTOKERATIN 18 - 3928795 3928795

References

Reference overviewPub Med ID
Transcriptional profiling of ectoderm specification to keratinocyte fate in human embryonic stem cells.
Tadeu, AM; Lin, S; Hou, L; Chung, L; Zhong, M; Zhao, H; Horsley, V
PloS one  10  e0122493  2015

Kivonat megmutatása
25849374 25849374
Generation of iPS Cells from Human Hair Follice Dermal Papilla Cells.
Muchkaeva, IA; Dashinimaev, EB; Artyuhov, AS; Myagkova, EP; Vorotelyak, EA; Yegorov, YY; Vishnyakova, KS; Kravchenko, IE; Chumakov, PM; Terskikh, VV; Vasiliev, AV
Acta naturae  6  45-53  2014

Kivonat megmutatása
24772326 24772326
Notch signaling represses p63 expression in the developing surface ectoderm.
Tadeu, AM; Horsley, V
Development (Cambridge, England)  140  3777-86  2013

Kivonat megmutatása
23924630 23924630
Effects of ligustrazine on ureteral obstruction-induced renal tubulointerstitial fibrosis.
Xiao-Peng Yuan,Long-Shan Liu,Qian Fu,Chang-Xi Wang
Phytotherapy research : PTR  26  2011

Kivonat megmutatása
22006851 22006851
Modulation of choroidal neovascularization by subretinal injection of retinal pigment epithelium and polystyrene microbeads.
Schmack, I; Berglin, L; Nie, X; Wen, J; Kang, SJ; Marcus, AI; Yang, H; Lynn, MJ; Kapp, JA; Grossniklaus, HE
Molecular vision  15  146-61  2009

Kivonat megmutatása Teljes cikk
19158960 19158960
Establishment of conditionally immortalized epithelial cell lines from the intestinal tissue of adult normal and transgenic mice.
Whitehead, RH; Robinson, PS
American journal of physiology. Gastrointestinal and liver physiology  296  G455-60  2009

Kivonat megmutatása Teljes cikk
19109407 19109407
[Extraembryonic endoderm stem cell lines from common voles of the genus Microtus].
A I Shevchenko,V V Demina,N A Mazurok,A I Zhelezova,Ia R Efremov,a G Shilov,A I Shevela,A V Belevantseva,V V Vlasov,S M Zakiian
Genetika  44  2008

Kivonat megmutatása
19137730 19137730
DeltaNp63 is essential for epidermal commitment of embryonic stem cells.
Alain Medawar,Thierry Virolle,Philippe Rostagno,Stéphanie de la Forest-Divonne,Karen Gambaro,Matthieu Rouleau,Daniel Aberdam
PloS one  3  2008

Kivonat megmutatása Teljes cikk
18927616 18927616
BMP-4 induces a Smad-dependent apoptotic cell death of mouse embryonic stem cell-derived neural precursors.
Gambaro, K; Aberdam, E; Virolle, T; Aberdam, D; Rouleau, M
Cell death and differentiation  13  1075-87  2005

Kivonat megmutatása
16311513 16311513
Identification of pulmonary Oct-4+ stem/progenitor cells and demonstration of their susceptibility to SARS coronavirus (SARS-CoV) infection in vitro.
Ling, TY; Kuo, MD; Li, CL; Yu, AL; Huang, YH; Wu, TJ; Lin, YC; Chen, SH; Yu, J
Proceedings of the National Academy of Sciences of the United States of America  103  9530-5  2005

Kivonat megmutatása
16772384 16772384

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Life Science Research > Antibodies and Assays > Primary Antibodies