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MABT901 Anti-Connexin 43 Antibody, C-terminal Antibody, clone P4G9

This mouse monoclonal Anti-Connexin 43, C-terminal, clone P4G9, Cat. No. MABT901 is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Immunoprecipitation for the detection of Connexin-43.

Anti-Connexin 43 Antibody, C-terminal Antibody, clone P4G9 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: Gja1, Cx43 UniProt Number: P08050
MABT901
100 μg  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RICC, IP, IH(P), IFMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT901
DescriptionAnti-Connexin 43 Antibody, C-terminal Antibody, clone P4G9
Alternate Names
  • Gap junction alpha-1 protein
  • CX43
  • Gap junction 43 kDa heart protein
Background InformationGap junction alpha-1 protein (UniProt: P08050; also known as Connexin-43, CX43, Gap junction 43 kDa heart protein) is encoded by the Gja1 (also known as Cxn-43) gene (Gene ID: 24392) in rat. The gap junction channels are dodecamers of transmembrane proteins of the connexin family. Connexins usually have a short half-life ( 1.5 - 5 h) and this short half-life is a key element of coupling regulation as it allows very dynamic and acute changes in gap junction regulation and gap junctional communication. In cardiomyocytes three types of connexins have been identified: Connexin 40, 43, and 45. Each of these connexins form channels with unique and specific electrophysiological properties. Connexin-43 is a multi-pass membrane protein and is the most ubiquitous connexin with widespread tissue expression pattern. Connexin-43 can be phosphorylated at multiple sites and phosphorylation is shown to regulate the kinetics of its trafficking, assembly, gating and turnover in a cell-cycle-stage specific manner. Phosphorylation at Ser325, Ser328 and Ser330 by casein kinase I is reported to modulate gap junction assembly while phosphorylation of Ser368 by protein kinase C gamma isoform leads to disassembly of gap junction plaques and inhibition of gap junction activity. During myocardial ischemia connexin-43 is shown to become dephosphorylated that allows gap junctions to be opened and convey the propagation of ischemia injury. In bladder smooth muscle cells, connexin-43 displays a circadian cycle with low levels during the sleep phase.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis mouse monoclonal Anti-Connexin 43, C-terminal, clone P4G9, Cat. No. MABT901 is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Immunoprecipitation for the detection of Connexin-43.
Key Applications
  • Immunocytochemistry
  • Immunoprecipitation
  • Immunohistochemistry (Paraffin)
  • Immunofluorescence
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Connexin 43 in rat heart tissue.

Immunofluorescence Analysis: A representative lot detected Connexin 43 in NRK cells (Courtesy of Joell Solan in Paul Lampe s Lab at the Fred Hutchinson Cancer Research Center, Seattle, WA).

Immunoprecipitation Analysis: A representative lot detected Connexin 43 in Immunoprecipitation applications (Sosinsky, G.E., et. al. (2007). Biochem J. 408(3):375-85).

Immunocytochemistry Analysis: A representative lot detected Connexin 43 in Immunocytochemistry applications (Norris, R.P., et. al. (2008). Development. 135(19):3229-38).

Immunocytochemistry Analysis: A representative lot detected Connexin 43 in Immunocytochemistry applications (Churko, J.M., et. al. (2010). Biochem J. 429(3):473-83).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal region of rat connexin-43.
EpitopeC-terminus
CloneP4G9
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone P4G9 specifically detects connexin 43 in human and rat heart. It targets an epitopw within 23 amino acids from the C-terminal end.
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteHuman, Rat, Mouse. Predicted to react with Bovine, Canine, Guinea Pig, Hamster, Porcine, Rabbit, and Rhesus Moneky based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Gja1
  • Cx43
Purification MethodProtein G purified
UniProt Number
Molecular Weight43.03 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human heart tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Connexin 43 in human heart tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABT901 04054839199356

Documentation

Anti-Connexin 43 Antibody, C-terminal Antibody, clone P4G9 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Connexin 43 Antibody, C-terminal Antibody, clone P4G9 Certificates of Analysis

TitleLot Number
Anti-Connexin 43, C-terminal, clone P4G9 - 3312985 3312985
Anti-Connexin 43, C-terminal, clone P4G9 - 3724634 3724634
Anti-Connexin 43, C-terminal, clone P4G9 - 3831306 3831306
Anti-Connexin 43, C-terminal, clone P4G9 - 4190448 4190448
Anti-Connexin 43, C-terminal, clone P4G9 Monoclonal Antibody Q2842208